J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 1, 2006

Papers In Press, published online ahead of print August 22, 2006
J. Lipid Res., doi:10.1194/jlr.M600136-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M600136-JLR200v1
47/11/2408    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brundert, M.
Right arrow Articles by Rinninger, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brundert, M.
Right arrow Articles by Rinninger, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on March 21, 2006
Revised on August 2, 2006
Accepted on August 22, 2006

Selective uptake of HDL cholesteryl esters and cholesterol efflux from mouse peritoneal macrophages independent of SR-BI

May Brundert, Joerg Heeren, Mukaddes Bahar-Bayansar, Anne Ewert, Kathryn J. Moore, and Franz Rinninger

Department of Medicine, Hospital Eppendorf, Hamburg, Hamburg 20246

Corresponding Author: Rinninger{at}uke.uni-hamburg.de

Objective - Scavenger receptor class B type I (SR-BI) mediates selective uptake of HDL cholesteryl esters (CE) and facilitates efflux of unesterified cholesterol. SR-BI expression in macrophages presumably plays a role in atherosclerosis. The role of SR-BI for selective CE uptake and cholesterol efflux in macrophages was explored. Methods and Results - Macrophages and HDL originated from wildtype (WT) or SR-BI Knockout (KO, homozygous) mice. For uptake, macrophages incubated in medium containing 125I-/[3H]-labeled HDL. For lipid removal, [3H]cholesterol efflux was analyzed using HDL as acceptor. Selective uptake of HDL CE ([3H]CEt – 125I-TC) was similar in WT and in SR-BI KO macrophages. Radiolabeled SR-BI KO-HDL yielded a lower rate of selective uptake compared to WT-HDL in WT and in SR-BI KO macrophages. Cholesterol efflux was similar in WT and in SR-BI KO cells using HDL as acceptor. SR-BI KO-HDL more efficiently promoted cholesterol removal compared to WT-HDL from both types of macrophages. Conclusions - Macrophages selectively take up HDL CE independently from SR-BI. Besides, in macrophages there is substantial cholesterol efflux which is not mediated by SR-BI. Therefore SR-BI-independent mechanisms mediate selective CE uptake and cholesterol removal. SR-BI KO-HDL is an inferior donor for selective CE uptake compared to WT-HDL whereas SR-BI KO-HDL more efficiently promotes cholesterol efflux.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
L. Yvan-Charvet, T. A. Pagler, N. Wang, T. Senokuchi, M. Brundert, H. Li, F. Rinninger, and A. R. Tall
SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL
J. Lipid Res., January 1, 2008; 49(1): 107 - 114.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.