J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 1, 2007

Papers In Press, published online ahead of print October 21, 2006
J. Lipid Res., doi:10.1194/jlr.M600155-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M600155-JLR200v1
48/1/9    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagayama, M.
Right arrow Articles by Gohara, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagayama, M.
Right arrow Articles by Gohara, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on April 4, 2006
Revised on September 26, 2006
Accepted on October 21, 2006

Temporal and spatial variations of lipid droplets during adipocyte division and differentiation

Masafumi Nagayama, Tsutomu Uchida, and Kazutoshi Gohara

Division of Applied Physics, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628

Corresponding Author: macci{at}eng.hokudai.ac.jp

By capturing time-lapse images of primary stromal-vascular cells (SVCs) derived from rat mesenteric adipose tissue, we revealed temporal and spatial variations of lipid droplets (LDs) in individual SVCs during adipocyte differentiation. Numerous small LDs (a few micrometers in diameter) appeared in the perinuclear region at an early stage of differentiation; subsequently, several LDs grew to more than 10 µm in diameter and occupied the cytoplasm. We have developed a method for the fluorescence staining of LDs in living adipocytes. Time-lapse observation of the stained cells at higher magnification showed that nascent LDs (several 100 nm in diameter) grew into small LDs while moving from lamellipodia to the perinuclear region. We also found that adipocytes are capable of division and they evenly distribute the LDs between two daughter cells. Immunofluorescence observations of lipid droplet-associated proteins revealed that such cell divisions of SVCs occurred even after LDs were coated with perilipin, suggesting that the “final” cell division during adipocyte differentiation occurs considerably later than that characterized in 3T3-L1 cells. Our time-lapse observations have provided a detailed account of the morphological changes that SVCs undergo during adipocyte division and differentiation.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. M. Goodman
The Gregarious Lipid Droplet
J. Biol. Chem., October 17, 2008; 283(42): 28005 - 28009.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
N. A. Ducharme and P. E. Bickel
Minireview: Lipid Droplets in Lipogenesis and Lipolysis
Endocrinology, March 1, 2008; 149(3): 942 - 949.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. J. Strissel, Z. Stancheva, H. Miyoshi, J. W. Perfield II, J. DeFuria, Z. Jick, A. S. Greenberg, and M. S. Obin
Adipocyte Death, Adipose Tissue Remodeling, and Obesity Complications
Diabetes, December 1, 2007; 56(12): 2910 - 2918.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.