Submitted on April 5, 2006
Revised on August 22, 2006
Accepted on August 25, 2006
Role of adipose differentiation-related protein in lung surfactant production: A reassessment
Pamela S. Mertz, Amy L. Magra, John S. Torday, and Constantine Londos
Laboratory of Cellular and Developmental Biology, NIDDK/NIH, Bethesda, MD 20892-8018
Corresponding Author: deanl{at}intra.niddk.nih.gov
ABSTRACT Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated that the droplet coat protein, ADRP, mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids (Schultz CJ, Torres E, Londos C, Torday JS, Am J Physiol Lung Cell Mol Physiol (283): L288-96, 2002. The present studies were designed to test the role of ADRP in this transfer with the use of ADRP-coated lipid droplets from CHO fibroblastic cells and a cultured human lung epithelial cell line. We find no role for ADRP in the lipid transfer and conclude that a lipase associated with the lipid droplets hydrolyzes their core triacylglycerols, releasing fatty acids that are taken up by the epithelial cells.
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