Submitted on April 5, 2006
Revised on October 3, 2006
Accepted on October 4, 2006
Novel anti-cholesterol monoclonal IgG antibodies as probes and potential modulators of membrane raft-dependent immune functions
Adrienn Bíró, László Cervenak, Andrea Balogh, András Lorincz, Katalin Uray, Anna Horváth, László Romics, János Matkó, George Füst, and Glória László
Immunology, Eotvos Lorand University, Budapest 1117
Corresponding Author: glorial{at}elte.hu
Natural autoantibodies against cholesterol are present in the sera of all healthy individuals, their function, production and regulation, however, is still unclear. Here we managed to produce two monoclonal anti-cholesterol IgG3 antibodies (ACHAs) by immunizing mice with cholesterol-rich liposomes. The new ACHAs are specific to cholesterol, to some structurally closely related 3
-OH sterols and reacted with human lipoproteins VLDL, LDL and HDL. They bound, usually with low avidity, to live human or murine lymphocyte and monocyte-macrophage cell lines, which was substantially enhanced by a moderate papain digestion of the cell surface, removing some protruding extracellular protein domains. Cell-bound ACHA strongly colocalized with markers of cholesterol-rich lipid rafts and caveolas at the cell surface and intracellularly with markers of the endoplasmic reticulum and Golgi complex. Data suggest that these IgG ACHAs may serve as probes of clustered cholesterol, e.g. different lipid rafts, in live cells and thus may also have an immunomodulatory potential.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
