J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 1, 2006

Papers In Press, published online ahead of print June 25, 2006
J. Lipid Res., doi:10.1194/jlr.M600185-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M600185-JLR200v1
47/9/1940    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, P. A.
Right arrow Articles by Crowther, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, P. A.
Right arrow Articles by Crowther, D. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on April 26, 2006
Revised on June 9, 2006
Accepted on June 25, 2006

Characterisation of the human patatin-like phospholipase family

Paul A. Wilson, Scott D. Gardner, Natalie M. Lambie, Stephane A. Commans, and Daniel J. Crowther

Bioinformatics Discovery & Analysis, GlaxoSmithKline R&D, Stevenage, England SG1 2NY

Corresponding Author: paw84313{at}gsk.com

Several publications have described biological roles for human patatin-like phospholipases (PNPLA) in the regulation of adipocyte differentiation. Here we report on the characterisation and expression profiling of ten human patatin-like phospholipases. A variety of Bioinformatics approaches were used to identify and characterise all patatin-like phospholipases encoded by the human genome. The genes described represent a divergent family, most with a highly conserved orthologue in several mammalian species. In silico characterisation predicts that two of the genes function as integral membrane proteins and are regulated by cAMP/cGMP. A structurally-guided protein alignment of the patatin-like domain identifies a number of conserved residues in all family members. Quantitative PCR was utilised to determine the expression profile of each family member. Affymetrix-based profiling of a human pre-adipocyte cell line identified several members that are differentially regulated during cell differentiation. Cumulative data suggests that patatin-like genes normally expressed at very low levels are induced in response to environmental signals. Given the observed conservation of the patatin fold and lipase motif in all human patatin-like phospholipases, a single nomenclature to describe a PNPLA family is proposed.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Eur J EndocrinolHome page
L. E Johansson, U. Lindblad, C. A Larsson, L. Rastam, and M. Ridderstrale
Polymorphisms in the adiponutrin gene are associated with increased insulin secretion and obesity
Eur. J. Endocrinol., November 1, 2008; 159(5): 577 - 583.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. C. Kienesberger, A. Lass, K. Preiss-Landl, H. Wolinski, S. D. Kohlwein, R. Zimmermann, and R. Zechner
Identification of an Insulin-regulated Lysophospholipase with Homology to Neuropathy Target Esterase
J. Biol. Chem., February 29, 2008; 283(9): 5908 - 5917.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. Mancuso, H. F. Sims, X. Han, C. M. Jenkins, S. P. Guan, K. Yang, S. H. Moon, T. Pietka, N. A. Abumrad, P. H. Schlesinger, et al.
Genetic Ablation of Calcium-independent Phospholipase A2{gamma} Leads to Alterations in Mitochondrial Lipid Metabolism and Function Resulting in a Deficient Mitochondrial Bioenergetic Phenotype
J. Biol. Chem., November 30, 2007; 282(48): 34611 - 34622.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.