| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print June 23, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Medicine/Endocrinology, University of Arkansas for Medical Sciences, Little Rock, AR 72205
Corresponding Author: kernphilipa{at}uams.edu
Metabolic syndrome and type 2 diabetes mellitus are associated with an increased number of macrophage cells that infiltrate white adipose tissue. Previously, we demonstrated that the treatment of impaired glucose tolerant (IGT) subjects with the PPAR agonist pioglitazone resulted in a decrease in macrophage number in adipose tissue. Here, adipose tissue samples from IGT subjects treated with pioglitazone were examined for apoptosis with TUNEL staining. TUNEL positive cells were identified and there was a significant 42% increase in TUNEL positive cells following pioglitazone treatment. Overlay experiments with anti-CD68 antibody demonstrated that most of the TUNEL positive cells were macrophages. To determine whether macrophage apoptosis was a direct or indirect effect of pioglitazone treatment, human THP1 cells were treated with pioglitazone in vitro, demonstrating increased TUNEL staining in a dose- and time-dependent manner. Furthermore, the appearance of the active proteolytic subunits of caspase-3 and caspase-9 were detected in cell lysate from THP1 cells and also increased in a dose- and time-dependent manner following pioglitazone. Pretreatment with a PPAR inhibitor, GW9662, prevented pioglitazone induction of the apoptotic pathway in THP1 cells. Differentiated human adipocytes did not show any significant increase in apoptosis after treatment in vitro with pioglitazone. These findings indicate that PPAR has distinct functions in different cell types in white adipose tissue, such that pioglitazone reduces macrophage infiltration by inducing apoptotic cell death specifically in macrophages through PPAR activation.
Accepted on June 23, 2006
Pioglitazone induces apoptosis of macrophages in human adipose tissue
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Yao-Borengasser, N. Rassouli, V. Varma, A. M. Bodles, N. Rasouli, R. Unal, B. Phanavanh, G. Ranganathan, R. E. McGehee Jr., and P. A. Kern Stearoyl-Coenzyme A Desaturase 1 Gene Expression Increases after Pioglitazone Treatment and Is Associated with Peroxisomal Proliferator-Activated Receptor-{gamma} Responsiveness J. Clin. Endocrinol. Metab., November 1, 2008; 93(11): 4431 - 4439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Espiritu and T. Mazzone Oxidative Stress Regulates Adipocyte Apolipoprotein E and Suppresses Its Expression in Obesity Diabetes, November 1, 2008; 57(11): 2992 - 2998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stienstra, C. Duval, S. Keshtkar, J. van der Laak, S. Kersten, and M. Muller Peroxisome Proliferator-activated Receptor {gamma} Activation Promotes Infiltration of Alternatively Activated Macrophages into Adipose Tissue J. Biol. Chem., August 15, 2008; 283(33): 22620 - 22627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Hausman, S. P. Poulos, T. D. Pringle, and M. J. Azain The influence of thiazolidinediones on adipogenesis in vitro and in vivo: Potential modifiers of intramuscular adipose tissue deposition in meat animals J Anim Sci, April 1, 2008; 86(14_suppl): E236 - E243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Varma, A. Yao-Borengasser, A. M. Bodles, N. Rasouli, B. Phanavanh, G. T. Nolen, E. M. Kern, R. Nagarajan, H. J. Spencer III, M.-J. Lee, et al. Thrombospondin-1 Is an Adipokine Associated With Obesity, Adipose Inflammation, and Insulin Resistance Diabetes, February 1, 2008; 57(2): 432 - 439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Schlezinger, J. K. Emberley, S. L. Bissonnette, and D. H. Sherr An L-Tyrosine Derivative and PPAR{gamma} Agonist, GW7845, Activates a Multifaceted Caspase Cascade in Bone Marrow B Cells Toxicol. Sci., July 1, 2007; 98(1): 125 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Kim, K. Tillison, S. Zhou, Y. Wu, and C. M. Smas The major facilitator superfamily member Slc37a2 is a novel macrophage- specific gene selectively expressed in obese white adipose tissue Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E110 - E120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kolak, H. Yki-Jarvinen, K. Kannisto, M. Tiikkainen, A. Hamsten, P. Eriksson, and R. M. Fisher Effects of Chronic Rosiglitazone Therapy on Gene Expression in Human Adipose Tissue in Vivo in Patients with Type 2 Diabetes J. Clin. Endocrinol. Metab., February 1, 2007; 92(2): 720 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ranganathan, R. Unal, I. Pokrovskaya, A. Yao-Borengasser, B. Phanavanh, B. Lecka-Czernik, N. Rasouli, and P. A. Kern The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment J. Lipid Res., November 1, 2006; 47(11): 2444 - 2450. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
