J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on February 1, 2007

Papers In Press, published online ahead of print November 8, 2006
J. Lipid Res., doi:10.1194/jlr.M600258-JLR200
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Submitted on June 14, 2006
Revised on October 18, 2006
Accepted on November 7, 2006

Inhibition of lipase activities by basic polysaccharide

Takahiro Tsujita, Hiroe Takaichi, Takeshi Takaku, Toshiya Sawai, Naoyuki Yoshida, and Jun Hiraki

Bioscience, Integrated Center for Sciences, Ehime University, Toon, Ehime 791-0295

Corresponding Author: tsujita{at}m.ehime-u.ac.jp

Basic polysaccharide strongly inhibited the hydrolysis of trioleoylglycerol (TO) emulsified with phosphatidylcholine (PC) and taurocholate by either pancreatic lipase or carboxylester lipase. Diethylaminoethyl (DEAE)-Sephadex dose-dependently inhibited the hydrolysis of trioleoylglycerol by pancreatic lipase and carboxylester lipase, however carboxymethyl (CM)-Sephadex and Sephadex G-50 did not inhibit the hydrolysis. Polydextrose (PD), a soluble polysaccharide, was a very weak inhibitor of pancreatic lipase. However, when a basic group, a DEAE-group, was attached to PD, lipase inhibition by DEAE-PD was increased and this was dependent on the substitution ratio of DEAE-groups. The number of positive charges per PD molecule is important in lipase inhibition. Similar substitution effects were observed with other basic groups, such as piperidinoethyl (PIPE), and 3-triethylamino-2-hydroxypropyl (TEAP). The natural basic polysaccharide, chitosan, also inhibited pancreatic lipase activity. Gel-filtration experiments suggested DEAE-PD did not bind strongly to pancreatic lipase. The effect of DEAE-PD on trioleoylglycerol hydrolysis by pancreatic lipase was studied using various emulsifiers: DEAE-PD (50 µg/ml) did not inhibit hydrolysis of trioleoylglycerol emulsified with arabic gum, phosphatidylserine (PS) or phosphatidic acid (PA). In vivo, oral administration of DEAE-PD to rats reduced the peak plasma triacylglycerol concentration and increased the fecal lipid excretion. These results suggest that basic polysaccharide is able to suppress dietary fat absorption from the small intestine by inhibiting pancreatic lipase activity.


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