Submitted on June 30, 2006
Revised on November 10, 2006
Accepted on November 18, 2006
Chronic high-fat diet affects the intestinal fat absorption and postprandial triglycerides levels in the mouse
Valerie Petit, Laurent Arnould, Pascal Martin, Marie-Claude Monnot, Thierry Pineau, Philippe Besnard, and Isabelle Niot
Physiology of Nutrition, ENSBANA, Dijon 21000
Corresponding Author: niot{at}u-bourgogne.fr
Impacts of a chronic fat over-consumption on the intestinal physiology and lipid metabolism remains elusive. It is unknown whether a fat-mediated adaptation of lipid absorption takes place. To address this issue, mice fed a high-fat diet (40%, m/m) were re-fed or not a control diet (3%, m/m) for 3 additive weeks. Despite daily lipid intake 7.7 fold higher than in controls, the faecal lipid output remained unchanged in mice fed the triglyceride-rich diet. In situ isolated jejunal loops revealed a greater [1-14C] linoleic acid uptake without triglyceride accumulation in mucosa, suggesting an increase in the lipid absorption capacity. An induction both in intestinal mitotic index and in the expression of genes involved in fatty acid uptake, trafficking and lipoprotein synthesis were found in high-fat diet mice. These changes was lipid-mediated since they were fully abolished in mice refed the control diet. Lipid load test performed in presence or absence of the lipoprotein lipase (LPL) inhibitor Tyloxapol showed a sustained blood triglycerides clearance in fat-fed mice likely due to intestinal modulation of LPL regulators (apolipoprotein C-II and C-III). These data demonstrate that a chronic high-fat diet greatly affect the intestinal physiology and body lipid use in the mouse.
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