J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2007 Originally published In Press as doi:10.1194/jlr.M600428-JLR200 on November 1, 2006

Papers In Press, published online ahead of print November 7, 2006
J. Lipid Res., doi:10.1194/jlr.M600428-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M600428-JLR200v1
M600428-JLR200v2
48/2/299    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koseki, M.
Right arrow Articles by Yamashita, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koseki, M.
Right arrow Articles by Yamashita, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on September 27, 2006
Revised on October 31, 2006
Accepted on November 1, 2006

Increased lipid rafts and accelerated lipopolysaccharide-induced tumor necrosis factor alpha secretion in Abca1-deficient macrophages

Masahiro Koseki, Ken-ichi Hirano, Daisaku Masuda, Chiaki Ikegami, Masaki Tanaka, Akemi Ota, Jose C. Sandoval, Yumiko Nakagawa-Toyama, Satoshi B. Sato, Toshihide Kobayashi, Yukiko Shimada, Yoshiko Ohno-Iwashita, Fumihiko Matsuura, Iichiro Shimomura, and Shizuya Yamashita

Metabolic Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871

Corresponding Author: koseki{at}imed2.med.osaka-u.ac.jp

Lipid rafts on the cell surface are believed to be very important as platforms for various cellular functions. The aim of the present study was to know whether defective lipid efflux may influence lipid rafts on the cell surface and their related cellular functions. We investigated macrophages (Mphi s) with defective lipid efflux from ATP-binding cassette transporter-A1 (Abca1)-deficient mice. Lipid rafts were evaluated by the following two novel probes: a protease-nicked and biotinylated derivative of perfringolysin O (BCtheta ) and a fluorescein ester of polyethylene glycol-derived cholesterol (fPEG-chol). Lipid rafts in Abca1-deficient (Abca1-KO) Mphi s were increased, as demonstrated by both probes. Moreover, activities of nuclear factor-kappa B (NFkappa B), mRNA and intracellular distribution, and secretion of tumor necrosis factor alpha (TNFalpha ) were examined following the stimulation by lipopolysaccharides (LPS). LPS-induced responses of activation of NF-kappa B and TNFalpha were more prompt and accelerated in the Abca1-KO Mphi s, compared with wild type Mphi s. Modification of lipid rafts by cyclodextrin and nystatin corrected the abnormal response, suggesting an association between the increased lipid rafts and abnormal TNFalpha secretion. We report here Abca1-KO Mphi s with defective lipid efflux exhibited the increased lipid rafts on the cell surface and the accelerated TNFalpha secretion.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CirculationHome page
L. Yvan-Charvet, C. Welch, T. A. Pagler, M. Ranalletta, M. Lamkanfi, S. Han, M. Ishibashi, R. Li, N. Wang, and A. R. Tall
Increased Inflammatory Gene Expression in ABC Transporter-Deficient Macrophages: Free Cholesterol Accumulation, Increased Signaling via Toll-Like Receptors, and Neutrophil Infiltration of Atherosclerotic Lesions
Circulation, October 28, 2008; 118(18): 1837 - 1847.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhu, J.-Y. Lee, J. M. Timmins, J. M. Brown, E. Boudyguina, A. Mulya, A. K. Gebre, M. C. Willingham, E. M. Hiltbold, N. Mishra, et al.
Increased Cellular Free Cholesterol in Macrophage-specific Abca1 Knock-out Mice Enhances Pro-inflammatory Response of Macrophages
J. Biol. Chem., August 22, 2008; 283(34): 22930 - 22941.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Nagao, K. Takahashi, K. Hanada, N. Kioka, M. Matsuo, and K. Ueda
Enhanced ApoA-I-dependent Cholesterol Efflux by ABCA1 from Sphingomyelin-deficient Chinese Hamster Ovary Cells
J. Biol. Chem., May 18, 2007; 282(20): 14868 - 14874.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Wang and J. F. Oram
Unsaturated fatty acids phosphorylate and destabilize ABCA1 through a protein kinase C {delta} pathway
J. Lipid Res., May 1, 2007; 48(5): 1062 - 1068.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.