J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2007 Originally published In Press as doi:10.1194/jlr.M600451-JLR200 on November 10, 2006

Papers In Press, published online ahead of print November 27, 2006
J. Lipid Res., doi:10.1194/jlr.M600451-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M600451-JLR200v1
M600451-JLR200v2
48/2/307    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gille, C.
Right arrow Articles by Orlikowsky, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gille, C.
Right arrow Articles by Orlikowsky, T. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on October 10, 2006
Revised on January 1, 1998
Accepted on November 9, 2006

Differential effect of surfactant and its saturated phosphatidylcholines on human blood macrophages

Christian Gille, Bärbel Spring, Wolfgang Bernhard, Caroline Gebhard, Denise Basile, Kirsten Lauber, Christian F. Poets, and Thorsten W. Orlikowsky

Neonatology, University Children´s Hospital Tuebingen, Tuebingen 72076

Corresponding Author: thorsten.orlikowsky{at}med.uni-tuebingen.de

Blood monocyte derived macrophages (Mphi ) invading the alveolus encounter pulmonary surfactant, a phospholipoprotein complex changing composition during lung development. We tested the hypothesis that characteristic phosphatidylcholine components differentially influence Mphi phenotype and function, as determined by phagocytosis of green-fluorescent-protein (gfp)-labelled Escherichia coli and alpha CD3 induced T cell proliferation. Human Mphi were exposed to surfactant (Curosurf), two of its characteristic phosphadidylcholine (PC)-components (dipalmitoyl-PC, palmitoylmyristoyl-PC), and an ubiquituous PC (palmitoyloleoyl-PC) as control. Interaction of Curosurf and PC species with Mphi was assessed using Lissamine-dilauroyl-phosphoethanolamie labelled liposomes. Curosurf and both saturated surfactant PC species down-regulated CD14 expression and up-regulated CD206. HLA-DR and CD80 were up-regulated by Curosurf and palmitoylmyristoyl-PC, while dipalmitoyl-PC showed no effect. The latter up-regulated TLR2 and TLR4-expression, while Curosurf and palmitoylmyristoyl-PC had no effect. PC species tested were incorporated in comparable amounts by Mphi . Curosurf and PC species inhibited phagocytosis of Escherichia coli. Scavenger receptors (CD36, CD68, SR-A and LOX-1) mRNA-expression was up-regulated by Curosurf, while PC species only up-regulated SR-A. Curosurf and palmitoylmyristoyl-PC inhibited alpha CD3-induced T cell proliferation by 50% while dipalmitoyl-PC and palmitoyloleoyl-PC showed no effect. These data identify individual surfactant PC species as modifiers of MF differentiation and suggest differential effects on innate and adaptive immune functions.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Integr. Comp. Biol.Home page
S. Orgeig, W. Bernhard, S. C. Biswas, C. B. Daniels, S. B. Hall, S. K. Hetz, C. J. Lang, J. N. Maina, A. K. Panda, J. Perez-Gil, et al.
The anatomy, physics, and physiology of gas exchange surfaces: is there a universal function for pulmonary surfactant in animal respiratory structures?
Integr. Comp. Biol., October 1, 2007; 47(4): 610 - 627.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.