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A more recent version of this article appeared on June 1, 2007

Papers In Press, published online ahead of print March 22, 2007
J. Lipid Res., doi:10.1194/jlr.M600472-JLR200
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Submitted on October 25, 2006
Revised on March 12, 2007
Accepted on March 22, 2007

Quantification and regulation of the subcellular distribution of bile acid CoA:amino acid N-acyltransferase activity in rat liver

Nathan A. Styles, Josie L. Falany, Stephen Barnes, and Charles N. Falany

Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294

Corresponding Author: charles.falany{at}ccc.uab.edu

Bile acid CoA:amino acid N-acyltranstransferase (BAT) is responsible for the amidation of bile acids with the amino acids glycine and taurine. To quantify the total BAT activity in liver subcellular organelles, livers from young adult male and female Sprague-Dawley rats were fractionated into multiple subcellular compartments. In male and female rats, 65-75% of total liver BAT activity was found in the cytosol, 15-17% in the peroxisomes, and 5-10% in the heavy mitochondrial fraction. After clofibrate treatment, male rats displayed an increase in peroxisomal BAT specific activity and a decrease in cytosolic BAT specific activity whereas females showed an opposite response. However, there was no overall change in BAT specific activity in whole liver homogenate. Treatment with rosiglitazone or cholestyramine had no effect on BAT activity in any subcellular compartment. These experiments indicate that the majority of BAT activity in the rat liver resides in the cytosol. Approximately 15% of BAT activity is present in the peroxisomal matrix. The data support the novel finding that clofibrate treatment does not directly regulate BAT activity but does alter the subcellular localization of BAT.


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E. M. Shonsey, S. M. Eliuk, M. S. Johnson, S. Barnes, C. N. Falany, V. M. Darley-Usmar, and M. B. Renfrow
Inactivation of human liver bile acid CoA:amino acid N-acyltransferase by the electrophilic lipid, 4-hydroxynonenal
J. Lipid Res., February 1, 2008; 49(2): 282 - 294.
[Abstract] [Full Text] [PDF]


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