Conjugated linoleic acid differently regulates adipogenesis in stromal vascular cells from subcutaneous adipose tissue and skeletal muscle of neonatal pigs

Xuan Zhou, Defa Li, Jingdong Yin, Jianjun Ni, Bing Dong, Jinxiao Zhang, and Min Du

College of Animal Science, China Agricultural University, Beijing 100094

Corresponding Author: yinjd{at}mafic.ac.cn

Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood. The objective of this study was to investigate the different effects of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular cells obtained from neonatal pigs. As shown in the present study, trans-10, cis-12 CLA decreased the expression of adipocyte-specific genes, as well as adipose precursor cell numbers and the accumulation of lipid in cultured SC adipose tissue stromal vascular cells. However, the cis-9, trans-11 CLA did not alter adipogenesis in SC cultures. On the other hand, both CLA isomers increased expression of adipocyte-specific genes in IM cultures, together with the increasing accumulation of lipid and oil red O-stained cells. Collectively, these data show CLA decreased SC adipose tissue but increased IM adipose tissue by different regulation of adipocyte-specific genes expression. These results suggest that adipogenesis in IM adipocytes differs from that in SC adipocytes.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

A. Di Giancamillo, R. Rossi, F. Vitari, G. Pastorelli, C. Corino, and C. Domeneghini
Dietary Conjugated Linoleic Acids Decrease Leptin in Porcine Adipose Tissue
J. Nutr., October 1, 2009; 139(10): 1867 - 1872.
[Abstract] [Full Text] [PDF]

G. J. Hausman, M. V. Dodson, K. Ajuwon, M. Azain, K. M. Barnes, L. L. Guan, Z. Jiang, S. P. Poulos, R. D. Sainz, S. Smith, et al.
BOARD-INVITED REVIEW: The biology and regulation of preadipocytes and adipocytes in meat animals
J Anim Sci, April 1, 2009; 87(4): 1218 - 1246.
[Abstract] [Full Text] [PDF]

Z. Zhan, D. Ou, X. Piao, S. W. Kim, Y. Liu, and J. Wang
Dietary Arginine Supplementation Affects Microvascular Development in the Small Intestine of Early-Weaned Pigs
J. Nutr., July 1, 2008; 138(7): 1304 - 1309.
[Abstract] [Full Text] [PDF]