| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print February 4, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
BPMS/NIA/NIH, Bethesda, MD 20892
Corresponding Author: mikii{at}mail.nih.gov
Background: Enzymes that convert a-linolenic acid (a-LNA, 18:3n-3) to docosahexaenoic acid (DHA, 22:6n-3) exist in the mammalian brain, but it is uncertain if the brain can upregulate this conversion during dietary deprivation of n-3 polyunsaturated fatty acids (PUFAs). Hypothesis: Brain synthesis-incorporation (conversion) coefficients of a-LNA into DHA will be upregulated in rats placed for 15 weeks, after weaning, on an n-3 PUFA deficient diet (0.2% a-LNA of total fatty acids, no DHA) compared with an adequate (4.5% a-LNA, no DHA) diet. Methods: Unanesthetized rats in each dietary group were infused intravenously with [1-14C]a-LNA, and their arterial plasma was collected until their brain was microwaved at 5 minutes, then chemically analyzed. Results: Rats fed the deficient compared with adequate diet had 30% less brain DHA and increased concentrations of arachidonic acid (AA, 20:4n-6) and docosapentaenoic acid (DPAn-6, 22:5n-6). The brain synthesis-incorporation coefficient of a-LNA to DPAn-3 (22:5n-3) was increased 3.4 fold, whereas coefficients to longer chain n-3 PUFAs including DHA were unchanged. Conclusions: The brain’s ability to synthesize DHA from a-LNA is not altered by n-3 PUFA deprivation. In the presence of a-LNA but not DHA in the diet, DHA converted from circulating a-LNA by the liver is the major source of brain DHA.
Revised on February 2, 2007
Accepted on February 4, 2007
Rate of synthesis of docosahexaenoic acid from
-linolenic acid by rat brain is not altered by dietary N-3 polyunsaturated fatty acid deprivation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. C. DeMar Jr., C. DiMartino, A. W. Baca, W. Lefkowitz, and N. Salem Jr. Effect of dietary docosahexaenoic acid on biosynthesis of docosahexaenoic acid from alpha-linolenic acid in young rats J. Lipid Res., September 1, 2008; 49(9): 1963 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Farias, M. Basselin, L. Chang, K. A. Heidenreich, S. I. Rapoport, and R. C. Murphy Formation of eicosanoids, E2/D2 isoprostanes, and docosanoids following decapitation-induced ischemia, measured in high-energy-microwaved rat brain J. Lipid Res., September 1, 2008; 49(9): 1990 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Igarashi, K. Ma, L. Chang, J. M. Bell, and S. I. Rapoport Rat heart cannot synthesize docosahexaenoic acid from circulating {alpha}-linolenic acid because it lacks elongase-2 J. Lipid Res., August 1, 2008; 49(8): 1735 - 1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Green, S. K. Orr, and R. P. Bazinet The emerging role of group VI calcium-independent phospholipase A2 in releasing docosahexaenoic acid from brain phospholipids J. Lipid Res., May 1, 2008; 49(5): 939 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Chen, D. W. L. Ma, J. H. Kim, H. T. J. Mount, and R. P. Bazinet The low density lipoprotein receptor is not necessary for maintaining mouse brain polyunsaturated fatty acid concentrations J. Lipid Res., January 1, 2008; 49(1): 147 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Igarashi, K. Ma, L. Chang, J. M. Bell, and S. I. Rapoport Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain J. Lipid Res., November 1, 2007; 48(11): 2463 - 2470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Levant, M. K. Ozias, and S. E. Carlson Diet (n-3) Polyunsaturated Fatty Acid Content and Parity Affect Liver and Erythrocyte Phospholipid Fatty Acid Composition in Female Rats J. Nutr., November 1, 2007; 137(11): 2425 - 2430. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
