J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 1, 2007

Papers In Press, published online ahead of print July 9, 2007
J. Lipid Res., doi:10.1194/jlr.M700039-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700039-JLR200v1
48/10/2133    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Llorente-Cortés, V.
Right arrow Articles by Badimon, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Llorente-Cortés, V.
Right arrow Articles by Badimon, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 24, 2007
Revised on June 1, 2007
Accepted on July 9, 2007

Adipocyte differentiation related protein (ADRP) is induced by LRP1-mediated aggregated LDL internalization in human vascular smooth muscle cells and macrophages

V. Llorente-Cortés, T. Royo, O. Juan-Babot, and L. Badimon

Cardiovascular Research Center, CSIC-ICCC-Hospital de la santa Creu i Sant Pau, Barcelona, Barcelona 08025

Corresponding Author: lbadimon{at}csic-iccc.santpau.es

Aggregated LDL (agLDL) are internalized by Low density lipoprotein receptor-related protein (LRP1) in vascular smooth muscle cells (VSMC) and macrophages (HMDM). AgLDL is, therefore, a potent inducer of massive intracellular cholesteryl ester (CE) accumulation in lipid droplets. The adipocyte-differentiation related protein (ADRP) has been found on the surface of lipid droplets. The objectives of this work were to analyze whether agLDL uptake modulates ADRP expression levels and whether the effect of agLDL internalization on ADRP expression depends on LRP1 in human VSMC and HMDM. AgLDL strongly upregulates ADRP mRNA (Real-time PCR) and protein expression (western blot) in human VSMC (mRNA: by 3.06-fold; protein: 8.58-fold) and HMDM (mRNA: by 3.5-fold; protein: by 3.71-fold). Treatment of VSMC and HMDM with small anti-LRP1 interfering RNA (siRNA-LRP1) lead to specific inhibition of LRP1 expression. siRNA-LRP1 treatment significantly reduced agLDL-induced ADRP overexpression in HMDM (by 69%) and in VSMC (by 53%). Immunohystochemical studies evidence a colocolocalization between ADRP/macrophages and ADRP/VSMC in advanced lipid-enriched atherosclerotic plaques. In summary, these results demonstrate that agLDL-LRP1 engagement induces ADRP overexpression in both HMDM and human VSMC and that ADRP is highly expressed in advanced lipid-enriched human atherosclerotic plaques. Therefore, LRP1-mediated agLDL uptake might play a pivotal role on vascular foam cell formation.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
J. Sendra, V. Llorente-Cortes, P. Costales, C. Huesca-Gomez, and L. Badimon
Angiotensin II upregulates LDL receptor-related protein (LRP1) expression in the vascular wall: a new pro-atherogenic mechanism of hypertension
Cardiovasc Res, June 1, 2008; 78(3): 581 - 589.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.