J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2007

Papers In Press, published online ahead of print March 30, 2007
J. Lipid Res., doi:10.1194/jlr.M700047-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700047-JLR200v1
48/6/1273    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Souza, S. C.
Right arrow Articles by Greenberg, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Souza, S. C.
Right arrow Articles by Greenberg, A. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 29, 2007
Revised on March 15, 2007
Accepted on March 29, 2007

Perilipin regulates the thermogenic actions of norepinephrine in brown adipose tissue

Sandra C. Souza, Marcelo A. Christoffolete, Miriam O. Ribeiro, Hideaki Miyoshi, Katherine J. Strissel, Zlatina S. Stancheva, Nicole H. Rogers, Tara M. D'Eon, James W. Perfield, Hitomi Imachi, Martin S. Obin, Antonio C. Bianco, and Andrew S. Greenberg

Obesity and Metabolism Laboratory, JM-USDA HNRCA at Tufts University, Boston, MA 02111

Corresponding Author: andrew.greenberg{at}tufts.edu

In response to cold, norepinephrine (NE)-induced triacylglycerol hydrolysis (lipolysis) in adipocytes of brown adipose tissue (BAT) provides fatty acid substrates to mitochondria for heat generation (adaptive thermogenesis). NE-induced lipolysis is mediated by protein kinase A (PKA)-dependent phosphorylation of perilipin, a lipid droplet-associated protein that is the major regulator of lipolysis. We investigated the role of perilipin PKA phosphorylation in BAT norepinephrine-stimulated thermogenesis using a novel mouse model in which a mutant form of perilipin, lacking all six PKA phosphorylation sites, is expressed in adipocytes of perilipin knockout mice. Here, we show that despite a normal mitochondrial respiratory capacity, NE-induced lipolysis is abrogated in the interscapular BAT (IBAT) of these mice. This lipolytic constraint is accompanied by a dramatic blunting (~70%) of the in vivo thermal response to norepinephrine. Thus, in the presence of perilipin, PKA-mediated perilipin phosphorylation is essential for NE-dependent lipolysis and full adaptive thermogenesis in BAT. In IBAT of Peri KO mice, elevated basal lipolysis due to the absence of perilipin is sufficient to support a rapid NE-stimulated temperature increase (~3.0 C) comparable to wild-type mice. This observation suggests that one or more NE-dependent mechanisms downstream of perilipin phosphorylation are required to initiate and/or sustain the IBAT thermal response.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.