Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 1, 2007

Papers In Press, published online ahead of print June 13, 2007
J. Lipid Res., doi:10.1194/jlr.M700051-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700051-JLR200v1
48/9/1966    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Natali, F.
Right arrow Articles by Gnoni, G. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Natali, F.
Right arrow Articles by Gnoni, G. V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 29, 2007
Revised on June 7, 2007
Accepted on June 11, 2007

Oleic acid is a potent inhibitor of fatty acid and cholesterol syntheses in C6 glioma cells

Francesco Natali, Luisa Siculella, Serafina Salvati, and Gabriele V. Gnoni

Scienze e Tecnologie Biologiche ed Ambientali, Università di Lecce, Lecce, Lecce 73100

Corresponding Author: gabriele.gnoni{at}unile.it

Glial cells play a pivotal role in brain fatty acid metabolism and membrane biogenesis. However, the potential regulation of lipogenesis and cholesterologenesis by fatty acids in glial cells has been barely investigated. Here we show that physiologically relevant concentrations of various saturated, monounsaturated and polyunsaturated fatty acids significantly reduce [1-14C]acetate incorporation into fatty acids and cholesterol in C6 cells. Oleic acid was the most effective in depressing lipogenesis and cholesterologenesis; a decreased label incorporation into cellular palmitic, stearic and oleic acid was detected, suggesting that enzymatic step(s) of de novo fatty acid biosynthesis was affected. To clarify this issue, the activities of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) were determined with an in situ digitonin-permeabilized cell assay after incubation of C6 cells with fatty acids. ACC activity was strongly reduced (~80%) by oleic acid, while no significant change in FAS activity was observed. Oleic acid also reduced the activity of 3-hydroxy-3-methylglutaryl Coenzyme A reductase (HMGCR). The inhibition of ACC and HMGCR activities is corroborated by the decrease in the ACC and HMGCR mRNA abundance and protein level. The down-regulation of ACC and HMGCR activity and expression by oleic acid could share in the reduced lipogenesis and cholesterologenesis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
P. M. Kgwatalala, E. M. Ibeagha-Awemu, A. F. Mustafa, and X. Zhao
Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows
J Dairy Sci, March 1, 2009; 92(3): 1220 - 1228.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement