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Papers In Press, published online ahead of print May 25, 2007
J. Lipid Res., doi:10.1194/jlr.M700113-JLR200
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Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, University Hospital, Malmö, Malmö S-205 02
Corresponding Author: Bjorn.Dahlback{at}med.lu.se
Apolipoprotein M (apoM) is a 188 amino acid, 25 kilodalton protein belonging to the lipocalin protein superfamily. Although predominantly associated with high-density lipoprotein, apoM is found in all major lipoprotein classes. In order to facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. The method has been used to investigate normal apoM variation and establish reference values for healthy individuals through measurement of 598 samples from the NOBIDA biobank. For women 18–49 years the reference interval for apoM was 0.58–1.18
Revised on May 25, 2007
Accepted on May 25, 2007
An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma
mol/L, whereas for women 50+ years and men the reference range was 0.61–1.30 mol/L. Correlation studies of apoM with twenty-six common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol (r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.
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