A rapid, small-scale procedure for the structural characterization of lipid A applied to Citrobacter and Bordetella strains:  discovery of a new structural element

doi:10.1194/jlr.M700193-JLR200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on November 1, 2007

Papers In Press, published online ahead of print August 16, 2007
J. Lipid Res., doi:10.1194/jlr.M700193-JLR200

This Article

Full Text (Accepted Manuscript)

All Versions of this Article:
M700193-JLR200v1
48/11/2419 most recent

Alert me when this article is cited

Alert me if a correction is posted

Services

Email this article to a friend

Similar articles in this journal

Similar articles in PubMed

Alert me to new issues of the journal

Download to citation manager

Request Permissions

Citing Articles

Citing Articles via HighWire

Citing Articles via Google Scholar

Google Scholar

Articles by Tirsoaga, A.

Articles by Novikov, A.

Search for Related Content

PubMed

PubMed Citation

Articles by Tirsoaga, A.

Articles by Novikov, A.

Social Bookmarking

What's this?

Submitted on April 23, 2007
Revised on August 14, 2007
Accepted on August 16, 2007

A rapid, small-scale procedure for the structural characterization of lipid A applied to Citrobacter and Bordetella strains: discovery of a new structural element

Alina Tirsoaga, Asmaa El hamidi, Malcolm B. Perry, Martine Caroff, and Alexey Novikov

IBBMC, Orsay 91405

Corresponding Author: alexey.novikov{at}u-psud.fr

Endotoxins (lipopolysaccharides, LPSs) are part of the outer cell membrane of Gram-negative bacteria; their biological activities are associated mainly with the lipid component (lipid A) and even more specifically with discrete aspects of their fine structure. The need for a rapid and small-scale analysis of lipid A motivated us to develop a procedure that combines direct isolation of lipids A from bacterial cells with sequential release of their ester-linked fatty acids by a mild alkali treatment followed by MALDI-MS analysis. The method avoids the multi-step LPS extraction procedure and lipid A isolation. The whole process can be performed in a working day and applied to lyophilized bacterial samples as small as 1 milligram. We illustrate the method by applying it to the analysis of lipids A of three species of Citrobacter which were found to be identical. On the other hand, when applied to two batches of Bordetella bronchiseptica strain 4650, it highlighted the presence in one of them, of hitherto unreported hexosamine residues substituting the lipid A phosphate groups, possibly a new camouflage opportunity to escape a host defense system.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

J. Lukasiewicz, W. Jachymek, T. Niedziela, L. Kenne, and C. Lugowski
Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides
J. Lipid Res., March 1, 2010; 51(3): 564 - 574.
[Abstract] [Full Text] [PDF]

P. Zhou, V. Chandan, X. Liu, K. Chan, E. Altman, and J. Li
Microwave-assisted sample preparation for rapid and sensitive analysis of H. pylori lipid A applicable to a single colony
J. Lipid Res., September 1, 2009; 50(9): 1936 - 1944.
[Abstract] [Full Text] [PDF]

A. El Hamidi, A. Novikov, D. Karibian, M. B. Perry, and M. Caroff
Structural characterization of Bordetella parapertussis lipid A
J. Lipid Res., May 1, 2009; 50(5): 854 - 859.
[Abstract] [Full Text] [PDF]

N. Marr, A. Tirsoaga, D. Blanot, R. Fernandez, and M. Caroff
Glucosamine Found as a Substituent of Both Phosphate Groups in Bordetella Lipid A Backbones: Role of a BvgAS-Activated ArnT Ortholog
J. Bacteriol., June 15, 2008; 190(12): 4281 - 4290.
[Abstract] [Full Text] [PDF]