J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 1, 2008

Papers In Press, published online ahead of print October 22, 2007
J. Lipid Res., doi:10.1194/jlr.M700355-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700355-JLR200v1
49/1/136    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Eck, M.
Right arrow Articles by Van Berkel, T. J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Eck, M.
Right arrow Articles by Van Berkel, T. J. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on August 9, 2007
Revised on October 19, 2007
Accepted on October 21, 2007

Scavenger receptor BI facilitates the metabolism of very-low-density lipoproteins in vivo

Miranda Van Eck, Menno Hoekstra, Ruud Out, I. Sophie T. Bos, J. Kar Kruijt, Reeni B. Hildebrand, and Theo J. C. Van Berkel

Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Leiden 2333CC

Corresponding Author: m.eck{at}lacdr.leidenuniv.nl

Scavenger receptor class B type I (SR-BI) functions as an HDL receptor that promotes the selective uptake of cholesteryl esters (CE). The physiological role of SR-BI in very-low-density lipoprotein (VLDL) metabolism, however, is largely unknown. SR-BI deficiency resulted in elevated VLDL cholesterol levels, both on chow diet and upon challenge with high cholesterol diets. The increase in VLDL cholesterol levels could not be explained by increased VLDL production, altered lipase activity, or reduced expression of the LDL receptor and LRP1, two important receptors for VLDL removal. To specifically elucidate the role of SR-BI in VLDL metabolism, the plasma clearance and hepatic uptake of 125I-betaVLDL were studied in SR-BI+/+ and SR-BI-/- mice. At 20 min after injection, 66±2% of the injected dose was taken up by the liver in SR-BI+/+ mice, as compared to only 22±4% (p=0.0007) in SR-BI-/- mice. Similar results were obtained using 3H-CE-betaVLDL, indicating that SR-BI does not mediate the selective uptake of cholesteryl esters from betaVLDL in vivo. In vitro studies using isolated hepatocytes established that the Bmax of 125I-betaVLDL binding was reduced from 469±30 ng/mg in SR-BI+/+ animals to 305±20 ng/mg (p=0.01) in SR-BI-/- mice. In agreement with the data obtained in vivo, also limited to no selective uptake of cholesteryl esters from betaVLDL was found in vitro. Interestingly, HDL effectively competed for the association of betaVLDL in the presence as well as in the absence of SR-BI, indicating a second common recognition site. In conclusion, in addition to its function as an HDL receptor, SR-BI plays an important physiological role in the metabolism of VLDL (remnants).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. A. Fenske, A. Yesilaltay, R. Pal, K. Daniels, A. Rigotti, M. Krieger, and O. Kocher
Overexpression of the PDZ1 Domain of PDZK1 Blocks the Activity of Hepatic Scavenger Receptor, Class B, Type I by Altering Its Abundance and Cellular Localization
J. Biol. Chem., August 8, 2008; 283(32): 22097 - 22104.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. Hu, C. C. van der Hoogt, S. M. S. Espirito Santo, R. Out, K. E. Kypreos, B. J. M. van Vlijmen, T. J. C. Van Berkel, J. A. Romijn, L. M. Havekes, K. W. van Dijk, et al.
The hepatic uptake of VLDL in lrp-ldlr-/-vldlr-/- mice is regulated by LPL activity and involves proteoglycans and SR-BI
J. Lipid Res., July 1, 2008; 49(7): 1553 - 1561.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.