|
 |
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print September 13, 2007
J. Lipid Res., doi:10.1194/jlr.M700359-JLR200
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215
Corresponding Author: deborah.brown{at}sunysb.edu
Although neutral lipid storage droplets are ubiquitous in eukaryotic cells, very little is known about how their synthesis and turnover are controlled. Adipocyte differentiation-related protein (ADRP; also known as adipophilin) is found on the surface of lipid droplets in most mammalian cell types. To learn how ADRP affects lipid storage, we stably expressed the protein in HEK 293 cells, which express little endogenous ADRP. As expected, ADRP was targeted to the surface of lipid droplets and caused an increase in triacylglycerol (TAG) mass under both basal and oleate-supplemented conditions. At least part of the increased mass resulted from a 50% decrease in the rate of TAG hydrolysis in ADRP-expressing cells. Furthermore, ADRP expression increased the fraction of total cellular TAG that was stored in lipid droplets. ADRP expression induced a striking decrease in association of adipose triglyceride lipase (ATGL) and TIP47 with lipid droplets, and also decreased lipid droplet association of several other unknown proteins. Transient expression of ADRP in two other cell lines also reduced lipid droplet association of catalytically-inactive ATGL. We conclude that reduced lipid droplet association of ATGL and/or other lipases may explain the decrease in TAG turnover observed in ADRP-expressing HEK 293 cells.
Revised on September 12, 2007
Accepted on September 12, 2007
Adipocyte differentiation-related protein reduces lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kaczocha, S. T. Glaser, J. Chae, D. A. Brown, and D. G. Deutsch Lipid Droplets Are Novel Sites of N-Acylethanolamine Inactivation by Fatty Acid Amide Hydrolase-2 J. Biol. Chem., January 22, 2010; 285(4): 2796 - 2806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, L. Hu, K. Dalen, H. Dorward, A. Marcinkiewicz, D. Russell, D. Gong, C. Londos, T. Yamaguchi, C. Holm, et al. Activation of Hormone-sensitive Lipase Requires Two Steps, Protein Phosphorylation and Binding to the PAT-1 Domain of Lipid Droplet Coat Proteins J. Biol. Chem., November 13, 2009; 284(46): 32116 - 32125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Minnaard, P. Schrauwen, G. Schaart, J. A. Jorgensen, E. Lenaers, M. Mensink, and M. K.C. Hesselink Adipocyte Differentiation-Related Protein and OXPAT in Rat and Human Skeletal Muscle: Involvement in Lipid Accumulation and Type 2 Diabetes Mellitus J. Clin. Endocrinol. Metab., October 1, 2009; 94(10): 4077 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. R. Meex, P. Schrauwen, and M. K. C. Hesselink Modulation of myocellular fat stores: lipid droplet dynamics in health and disease Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2009; 297(4): R913 - R924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Sapiro, M. T. Mashek, A. S. Greenberg, and D. G. Mashek Hepatic triacylglycerol hydrolysis regulates peroxisome proliferator-activated receptor {alpha} activity J. Lipid Res., August 1, 2009; 50(8): 1621 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bezaire, A. Mairal, C. Ribet, C. Lefort, A. Girousse, J. Jocken, J. Laurencikiene, R. Anesia, A.-M. Rodriguez, M. Ryden, et al. Contribution of Adipose Triglyceride Lipase and Hormone-sensitive Lipase to Lipolysis in hMADS Adipocytes J. Biol. Chem., July 3, 2009; 284(27): 18282 - 18291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bourdon, S. Hojna, M. Jordan, J. Berube, V. Kren, M. Pravenec, P. Liu, S. Arab, and Z. Pausova Genetic locus on rat chromosome 20 regulates diet-induced adipocyte hypertrophy: a microarray gene expression study Physiol Genomics, June 10, 2009; 38(1): 63 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Eastman, M. Yassaee, and P. D. Bieniasz A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover J. Cell Biol., March 23, 2009; 184(6): 881 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zechner, P. C. Kienesberger, G. Haemmerle, R. Zimmermann, and A. Lass Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores J. Lipid Res., January 1, 2009; 50(1): 3 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Prentki and S. R. M. Madiraju Glycerolipid Metabolism and Signaling in Health and Disease Endocr. Rev., October 1, 2008; 29(6): 647 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Imanishi, W. Sun, T. Maeda, A. Maeda, and K. Palczewski Retinyl Ester Homeostasis in the Adipose Differentiation-related Protein-deficient Retina J. Biol. Chem., September 5, 2008; 283(36): 25091 - 25102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bell, H. Wang, H. Chen, J. C. McLenithan, D.-W. Gong, R.-Z. Yang, D. Yu, S. K. Fried, M. J. Quon, C. Londos, et al. Consequences of Lipid Droplet Coat Protein Downregulation in Liver Cells: Abnormal Lipid Droplet Metabolism and Induction of Insulin Resistance Diabetes, August 1, 2008; 57(8): 2037 - 2045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Cocchiaro, Y. Kumar, E. R. Fischer, T. Hackstadt, and R. H. Valdivia From the Cover: Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole PNAS, July 8, 2008; 105(27): 9379 - 9384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schweiger, G. Schoiswohl, A. Lass, F. P. W. Radner, G. Haemmerle, R. Malli, W. Graier, I. Cornaciu, M. Oberer, R. Salvayre, et al. The C-terminal Region of Human Adipose Triglyceride Lipase Affects Enzyme Activity and Lipid Droplet Binding J. Biol. Chem., June 20, 2008; 283(25): 17211 - 17220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Keller, J. T. Petrie, P. De Rose, I. Gerin, W. S. Wright, S.-H. Chiang, A. R. Nielsen, C. P. Fischer, B. K. Pedersen, and O. A. MacDougald Fat-specific Protein 27 Regulates Storage of Triacylglycerol J. Biol. Chem., May 23, 2008; 283(21): 14355 - 14365. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |