Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 1, 2008 Originally published In Press as doi:10.1194/jlr.M700503-JLR200 on February 5, 2008

Papers In Press, published online ahead of print February 4, 2008
J. Lipid Res., doi:10.1194/jlr.M700503-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M700503-JLR200v1
M700503-JLR200v2
49/5/995    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by VanRollins, M.
Right arrow Articles by Morrow, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by VanRollins, M.
Right arrow Articles by Morrow, J. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on November 2, 2007
Revised on January 28, 2008
Accepted on February 4, 2008

F2-dihomo-isoprostanes arise from free radical attack on adrenic acid

Mike VanRollins, Randall L. Woltjer, Huiyong Yin, and Jason D. Morrow

Pathology, University of Washington, Seattle, WA 98101

Corresponding Author: tmontine{at}u.washington.edu

Unlike F4-Neuroprostanes (NeuroPs) that are relatively selective in vivo markers of oxidative damage to neuronal membranes, there currently is no method to assess the extent of free radical damage to myelin with relative selectively. The polyunsaturated fatty acid adrenate (AdA) is susceptible to free radical attack, and, at least in primates, is concentrated in myelin within white matter. Here we characterized oxidation products of AdA as potential markers of free radical damage to myelin in human brain. Unesterified AdA was reacted with a free radical initiator to yield products (F2-Dihomo-IsoPs) that were 28 Da larger but otherwise closely resembled F2-Isoprostanes (IsoPs), which are generated by free radical attack on arachidonic acid (AA). Phospholipids derived from human cerebral gray matter, white matter, and myelin similarly oxidized ex vivo showed that the ratio of esterified F2-Dihomo-IsoPs to F4-NeuroPs was approximately 10-fold greater in myelin- than gray matter-derived phospholipids. Finally, we showed that F2-Dihomo-IsoPs are significantly increased in white matter samples from patients with Alzheimer’s disease. We propose that F2-Dihomo-IsoPs may serve as quantitative in vivo biomarkers of free radical damage to myelin from primate white matter.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
L. J. Roberts II and G. L. Milne
Isoprostanes
J. Lipid Res., April 1, 2009; 50(Supplement): S219 - S223.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement