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Papers In Press, published online ahead of print April 22, 2008
J. Lipid Res., doi:10.1194/jlr.M800129-JLR200
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Submitted on March 10, 2008
Revised on April 10, 2008
Accepted on April 22, 2008

Identification of a novel glycerophosphocholine acyltransferase activity and a new pathway for phosphatidylcholine biosynthesis in Saccharomyces cerevisiae

Kjell Stålberg, Andrea C. Neal, Hans Ronne, and Ulf Ståhl

Plant Breeding and Biotechnology, Swedish University for Agricultural Sciences, Alnarp 23053

Corresponding Author: ulf.stahl{at}vbsg.slu.se

Turnover of phospholipids in the yeast Saccharomyces cerevisiae generates intracellular glycerophosphocholine (GPC). Here we show that GPC can be reacylated in an acyl-CoA dependent reaction by yeast microsomal membranes. The lysophosphatidylcholine that is formed in this reaction is efficiently further acylated to phosphatidylcholine (PC) by yeast microsomes, thus providing a new pathway for PC biosynthesis which can either recycle endogenously generated GPC or utilize externally provided GPC. Genetic and biochemical evidence suggest that this new enzymatic activity, which we call GPC acyltransferase (GPCAT), is not mediated by any of the previously known acyltransferases in yeast. The GPCAT activity has an apparent Vmax of 8.7 nmol/min/mg protein and an apparent Km of 2.5 mM. It has a neutral pH optimum, similar to yeast glycerol- 3-phosphate acyltransferase, but differs from the latter in being more heat stable. The GPCAT activity is sensitive to N-ethylmaleimide, phenanthroline and Zn2+ ions. In vivo experiments showed that PC is efficiently labeled when yeast cells are fed with [3H]choline-GPC, and that this reaction occurs also in pct1 knockout strains, where de novo synthesis of PC by the CDP-choline pathway is blocked. This suggests that GPCAT can provide an alternative pathway for PC biosynthesis in vivo.


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