Submitted on October 22, 2008
Revised on April 30, 2009
Accepted on May 8, 2009
Colocalization of prostaglandin F2
receptor FP and prostaglandin F synthase-I in the spinal cord
Toshiko Suzuki-Yamamoto, Kazunori Toida, Yukihiko Sugimoto, and Kazunori Ishimura
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja 719-1197
Corresponding Author: toshiko{at}fhw.oka-pu.ac.jp
Prostaglandin F2a is synthesized by prostaglandin F synthase, which exists in two types, prostaglandin F synthase I (PGFS I) and prostaglandin F synthase II (PGFS II). Prostaglandin F2a binds to its specific receptor, FP. Our previous immunohistochemical study showed the distinct localization of prostaglandin F synthases in rat spinal cord. PGFS I exists in neuronal somata and dendrites in the gray matter, and PGFS II exists in ependymal cells and tanycytes surrounding the central canal. Both enzymes are also present in endothelial cells of blood vessels in the white and gray substances of the spinal cord. In the present study, we found that FP localizes in neuronal somata and dendrites, but not in ependymal cells, tanycytes, or endothelial cells. Immunohistochemical analysis of serial sections showed the colocalization of FP and PGFS I. FP immunoreactivity was intense in spinal laminae I and II of the dorsal horn, a connection site of pain transmission, and was similar to that of PGFS I in neuronal elements. These findings suggest that prostaglandin F2a synthesized in the neuronal somata and dendrites exert an autocrine action there.
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