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Fig. 5. IC₅₀ determination. Fluconazole inhibition of CA 14DM, H 14DM and mutant activities based on IC₅₀ (µM). Values are determined for 50 µM DHL conversion at 37°C in presence of increasing concentrations of fluconazole and expressed as the percent of activity with fluconazole versus activity without fluconazole (log scale). * Up to 10 mM limit of solubility for fluconazole, H 14DM is still fully active. Error bars represent standard deviation.

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