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Journal of Lipid Research, Vol. 11, 583-595, November 1970
Centre National de Transfusion Sanguine, Paris, France; and Department of Medicine, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461
Procedures are described for the isolation of lipoproteins from human serum by precipitation with polyanions and divalent cations. A mixture of low and very low density lipoproteins can be prepared without ultracentrifugation by precipitation with heparin and either MnCl2 alone or MgCl2 plus sucrose. In both cases the precipitation is reversible, selective, and complete. The highly concentrated isolated lipoproteins are free of other plasma proteins as judged by immunological and electrophoretic methods. The low density and very low density lipoproteins can then be separated from each other by ultracentrifugation. The advantage of the method is that large amounts of lipoproteins can be prepared with only a single preparative ultracentrifugation. Polyanions other than heparin may also be used; when the precipitation of the low and very low density lipoproteins is achieved with dextran sulfate and MnCl2, or sodium phosphotungstate and MgCl2, the high density lipoproteins can subsequently be precipitated by increasing the concentrations of the reagents. These lipoproteins, containing small amounts of protein contaminants, are further purified by ultracentrifugation at d 1.22. With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum. Supplementary key words heparin • dextran sulfate • sodium phosphotungstate • divalent cations • sucrose • immunology • ultracentrifugation • zonal electrophoresis
Submitted on April 27, 1970
Copyright © 1970 by Lipid Research, Inc.
Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions
Accepted on August 13, 1970
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