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Journal of Lipid Research, Vol. 44, 2065-2072, November 2003
Copyright © 2003 by American Society for Biochemistry and Molecular Biology
* Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, OX3 7LJ, United Kingdom
Lipoprotein and Atherosclerosis Research Group, Department of Pathology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada
Institute of Human Nutrition, University of Southampton, Southampton, SO16 6YD, United Kingdom
1 To whom correspondence should be addressed. e-mail: fredrik.karpe{at}oxlip.ox.ac.uk
Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 ± 2.2 years, body mass index 22.1 ± 2.3 kg/m2) were fed a mixed meal containing 30 g fish oil and 600 mg [1-13C]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apolipoprotein B-100. The fatty acid composition of lipoproteins was analyzed by gas chromatography/mass spectrometry. [1-13C]palmitic acid started to appear in VLDL TG 3 h after meal intake, and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Approximately 20% of dietary fatty acids entered the VLDL TG pool 6 h after meal intake. DHA was clearly overincorporated into this pool compared with [1-13C]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the nonesterified fatty acid pool.
In summary, the contribution of dietary fatty acids to early postprandial VLDL TG is substantial. The role of DHA in VLDL TG production will require further investigation.
Abbreviations: apoB, apolipoprotein B; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; RE, retinyl ester; TG, triacylglycerol
Supplementary key words chylomicron • apolipoprotein B • immunoaffinity chromatography • triacylglycerol • n-3 polyunsaturated fatty acids • palmitic acid • stable isotopes
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