HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M200393-JLR200v1 44/3/533    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Igel, M. Articles by von Bergmann, K. Search for Related Content PubMed PubMed Citation Articles by Igel, M. Articles by von Bergmann, K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 44, 533-538, March 2003
Copyright © 2003 by Lipid Research, Inc.

Comparison of the intestinal uptake of cholesterol, plant sterols, and stanols in mice

Michael Igel, Uwe Giesa, Dieter Lütjohann and Klaus von Bergmann¹

Department of Clinical Pharmacology, University of Bonn, Germany

¹ To whom correspondence should be addressed. e-mail: vonbergmann{at}uni-bonn.de

The recent identification of the aberrant transport proteins ABCG5 and ABCG8 resulting in sitosterolemia suggests that intestinal uptake of cholesterol is an unselective process, and that discrimination between cholesterol and plant sterols takes place at the level of sterol efflux from the enterocyte. Although plant sterols are structurally very similar to cholesterol, differing only in their side chain length, they are absorbed from the intestine to a markedly lower extent. In order to further evaluate the process of discrimination, three different sterols (cholesterol, campesterol, sitosterol) and their corresponding 5-stanols (cholestanol, campestanol, sitostanol) were compared concerning their concentration in the proximal small intestine, in serum, and in bile after a single oral dose of deuterated compounds.

The data obtained support the hypothesis that i) the uptake of sterols and stanols is an extremely rapid process, ii) discrimination probably takes place on the level of reverse transport back into the gut lumen, iii) plant stanols are taken up, but not absorbed to a measurable extent, and iv) the process of discrimination probably also exists at the level of biliary excretion. The range of structural alterations that decrease intestinal absorption and increase biliary excretion is: 1) campesterol, 2) cholestanol-sitosterol, and 3) campestanol-sitostanol.

Supplementary key words phytosterols • phytostanols • stable isotope • gas chromatography-mass spectrometry • ABC-transporters

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. G. Elkin and E. S. Lorenz Feeding laying hens a bioavailable soy sterol mixture fails to enrich their eggs with phytosterols or elicit egg yolk compositional changes Poult. Sci., January 1, 2009; 88(1): 152 - 158. [Abstract] [Full Text] [PDF]

				J. K. Kruit, A. L. Drayer, V. W. Bloks, N. Blom, S. G. Olthof, P. J. J. Sauer, G. de Haan, I. P. Kema, E. Vellenga, and F. Kuipers Plant Sterols Cause Macrothrombocytopenia in a Mouse Model of Sitosterolemia J. Biol. Chem., March 7, 2008; 283(10): 6281 - 6287. [Abstract] [Full Text] [PDF]

				J. J. Clarenbach, M. Reber, D. Lutjohann, K. von Bergmann, and T. Sudhop The lipid-lowering effect of ezetimibe in pure vegetarians J. Lipid Res., December 1, 2006; 47(12): 2820 - 2824. [Abstract] [Full Text] [PDF]

				E. J. Westover, X. Lin, T. E. Riehl, L. Ma, W. F. Stenson, D. F. Covey, and R. E. Ostlund Jr. Rapid transient absorption and biliary secretion of enantiomeric cholesterol in hamsters J. Lipid Res., November 1, 2006; 47(11): 2374 - 2381. [Abstract] [Full Text] [PDF]

				T. Plosch, J. N. van der Veen, R. Havinga, N. C. A. Huijkman, V. W. Bloks, and F. Kuipers Abcg5/Abcg8-independent pathways contribute to hepatobiliary cholesterol secretion in mice Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G414 - G423. [Abstract] [Full Text] [PDF]

				K. M. Thelen, K. M. Rentsch, U. Gutteck, M. Heverin, M. Olin, U. Andersson, A. von Eckardstein, I. Bjorkhem, and D. Lutjohann Brain Cholesterol Synthesis in Mice Is Affected by High Dose of Simvastatin but Not of Pravastatin J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1146 - 1152. [Abstract] [Full Text] [PDF]

				J. Plat, J. A. Nichols, and R. P. Mensink Plant sterols and stanols: effects on mixed micellar composition and LXR (target gene) activation J. Lipid Res., November 1, 2005; 46(11): 2468 - 2476. [Abstract] [Full Text] [PDF]

				H. Tomoyori, Y. Kawata, T. Higuchi, I. Ichi, H. Sato, M. Sato, I. Ikeda, and K. Imaizumi Phytosterol Oxidation Products Are Absorbed in the Intestinal Lymphatics in Rats but Do Not Accelerate Atherosclerosis in Apolipoprotein E-Deficient Mice J. Nutr., July 1, 2004; 134(7): 1690 - 1696. [Abstract] [Full Text]

				F. J. Field, E. Born, and S. N. Mathur LXR/RXR ligand activation enhances basolateral efflux of {beta}-sitosterol in CaCo-2 cells J. Lipid Res., May 1, 2004; 45(5): 905 - 913. [Abstract] [Full Text] [PDF]

				L. Yu, K. von Bergmann, D. Lutjohann, H. H. Hobbs, and J. C. Cohen Selective sterol accumulation in ABCG5/ABCG8-deficient mice J. Lipid Res., February 1, 2004; 45(2): 301 - 307. [Abstract] [Full Text] [PDF]