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Journal of Lipid Research, Vol. 44, 612-620, March 2003
Copyright © 2003 by Lipid Research, Inc.

Red blood cell fatty acid ethyl esters

Catherine A. Best^*,, Joanne E. Cluette-Brown^*,, Miho Teruya^*,, Ami Teruya^*, and Michael Laposata^1,*,

^* Division of Laboratory Medicine, Department of Pathology, Massachusetts General Hospital, Boston, MA
Harvard Medical School, Boston, MA

¹ To whom correspondence should be addressed. e-mail: mlaposata{at}partners.org

Although alcohol abuse is known to cause an array of ethanol-induced red blood cell (RBC) abnormalities, the underlying molecular mechanisms remain poorly understood. Fatty acid ethyl esters (FAEEs) are toxic, nonoxidative ethanol metabolites that have been found in blood, plasma, and tissues. Because FAEEs have been shown to be incorporated into phospholipid bilayers, we conducted a controlled ethanol intake study to test the hypothesis that FAEEs accumulate and persist within RBCs following ethanol ingestion. We demonstrated that RBC FAEEs account for approximately 5% to 20% of total whole-blood FAEEs, and that the fatty acid composition of FAEEs in RBCs and plasma are different and vary differently over time.

These data indicate that a significant percentage of FAEEs in the blood is associated with RBCs and that the metabolism of RBC FAEEs and that of plasma FAEEs (bound to albumin or lipoproteins) are largely independent.

Abbreviations: AIC, akaike information criteria; E16:0, ethyl palmitate; E18:0, ethyl stearate; E18:1, ethyl oleate; E18:2, ethyl linoleate; FAEE, fatty acid ethyl ester; GC-MS, gas liquid chromatography-mass spectrometry; RBC, red blood cell; SPE, solid-phase extraction; WBC, white blood cell

Supplementary key words ethanol • red blood cell membranes • alcohol monitoring • alcoholism • alcohol • addiction

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