| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Lipid Research, Vol. 44, 1071-1079, June 2003
Copyright © 2003 by Lipid Research, Inc.
Thematic Review |
,
,**
* Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St. Louis, MO 63110
Departments of Medicine, Washington University School of Medicine, St. Louis, MO 63110
Pharmacology & Molecular Biology, Washington University School of Medicine, St. Louis, MO 63110
** Chemistry, Washington University School of Medicine, St. Louis, MO 63110
1 To whom correspondence should be addressed. e-mail: xianlin{at}pcg.wustl.edu
Lipidomics is a rapidly expanding research field in which multiple techniques are utilized to quantitate the hundreds of chemically distinct lipids in cells and determine the molecular mechanisms through which they facilitate cellular function. Recent developments in electrospray ionization mass spectrometry (ESI/MS) have made possible, for the first time, the precise identification and quantification of alterations in a cell's lipidome after cellular perturbations. This review provides an overview of the essential role of ESI/MS in lipidomics, presents a broad strategy applicable for the generation of lipidomes directly from cellular extracts of biological samples by ESI/MS, and summarizes salient examples of strategies utilized to conquer the lipidome in physiologic signaling as well as pathophysiologically relevant disease states. Because of its unparalleled sensitivity, specificity, and efficiency, ESI/MS has provided a critical bridge to generate highly accurate data that fingerprint cellular lipidomes to facilitate insight into the functional role of subcellular membrane compartments and microdomains in mammalian cells.
We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease.
Abbreviations: AA, arachidonic acid; AD, Alzheimer's disease; ESI, electrospray ionization; FFA, free fatty acid; GalC, galactocerebroside; MS, mass spectrometry; NL, neutral loss; PC, choline glycerophospholipid; PE, ethanolamine glycerophospholipid; PlsEtn, plasmenylethanolamine; PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdGro, phosphatidylglycerol; PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; SM, sphingomyelin; TAG, triacylglycerol
Supplementary key words Alzheimer's disease • diabetes • electrospray ionization mass spectrometry • phospholipid • plasmalogen • platelets • tandem mass spectrometry • triacylglycerol
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. R. Nealon, S. J. Blanksby, T. W. Mitchell, and P. L. Else Systematic differences in membrane acyl composition associated with varying body mass in mammals occur in all phospholipid classes: an analysis of kidney and brain J. Exp. Biol., October 1, 2008; 211(19): 3195 - 3204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Hou, H. Zhou, F. Elisma, S. A. L. Bennett, and D. Figeys Technological developments in lipidomics Brief Funct Genomic Proteomic, September 19, 2008; (2008) eln042v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Pruett, A. Bushnev, K. Hagedorn, M. Adiga, C. A. Haynes, M. C. Sullards, D. C. Liotta, and A. H. Merrill Jr. Thematic Review Series: Sphingolipids. Biodiversity of sphingoid bases ("sphingosines") and related amino alcohols J. Lipid Res., August 1, 2008; 49(8): 1621 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Nassir, B. Wilson, X. Han, R. W. Gross, and N. A. Abumrad CD36 Is Important for Fatty Acid and Cholesterol Uptake by the Proximal but Not Distal Intestine J. Biol. Chem., July 6, 2007; 282(27): 19493 - 19501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Moulson, M.-H. Lin, J. M. White, E. P. Newberry, N. O. Davidson, and J. H. Miner Keratinocyte-specific Expression of Fatty Acid Transport Protein 4 Rescues the Wrinkle-free Phenotype in Slc27a4/Fatp4 Mutant Mice J. Biol. Chem., May 25, 2007; 282(21): 15912 - 15920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Nichols, B. M. Ham, J. J. Nichols, C. Ziegler, and K. B. Green-Church Identification of Fatty Acids and Fatty Acid Amides in Human Meibomian Gland Secretions Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 34 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hidaka, N. Hanyu, M. Sugano, K. Kawasaki, K. Yamauchi, and T. Katsuyama Analysis of Human Serum Lipoprotein Lipid Composition Using MALDI-TOF Mass Spectrometry Ann. Clin. Lab. Sci., January 1, 2007; 37(3): 213 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Esch, P. Tamura, A. A. Sparks, M. R. Roth, S. P. Devaiah, E. Heinz, X. Wang, T. D. Williams, and R. Welti Rapid characterization of the fatty acyl composition of complex lipids by collision-induced dissociation time-of-flight mass spectrometry J. Lipid Res., January 1, 2007; 48(1): 235 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Jiang and X. Han Characterization and direct quantitation of sphingoid base-1-phosphates from lipid extracts: a shotgun lipidomics approach J. Lipid Res., August 1, 2006; 47(8): 1865 - 1873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Han, K. Yang, J. Yang, H. Cheng, and R. W. Gross Shotgun lipidomics of cardiolipin molecular species in lipid extracts of biological samples J. Lipid Res., April 1, 2006; 47(4): 864 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yan, C. M. Jenkins, X. Han, D. J. Mancuso, H. F. Sims, K. Yang, and R. W. Gross The Highly Selective Production of 2-Arachidonoyl Lysophosphatidylcholine Catalyzed by Purified Calcium-independent Phospholipase A2{gamma}: IDENTIFICATION OF A NOVEL ENZYMATIC MEDIATOR FOR THE GENERATION OF A KEY BRANCH POINT INTERMEDIATE IN EICOSANOID SIGNALING J. Biol. Chem., July 22, 2005; 280(29): 26669 - 26679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Han, K. Yang, H. Cheng, K. N. Fikes, and R. W. Gross Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization J. Lipid Res., July 1, 2005; 46(7): 1548 - 1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Lee, A. Lesimple, A. Larsen, O. Mamer, and J. Genest ESI-MS quantitation of increased sphingomyelin in Niemann-Pick disease type B HDL J. Lipid Res., June 1, 2005; 46(6): 1213 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Chiu, A. Kovacs, R. M. Blanton, X. Han, M. Courtois, C. J. Weinheimer, K. A. Yamada, S. Brunet, H. Xu, J. M. Nerbonne, et al. Transgenic Expression of Fatty Acid Transport Protein 1 in the Heart Causes Lipotoxic Cardiomyopathy Circ. Res., February 4, 2005; 96(2): 225 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Han and H. Cheng Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics J. Lipid Res., January 1, 2005; 46(1): 163 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. W. Mitchell, N. Turner, A. J. Hulbert, P. L. Else, J. A. Hawley, J. S. Lee, C. R. Bruce, and S. J. Blanksby Exercise alters the profile of phospholipid molecular species in rat skeletal muscle J Appl Physiol, November 1, 2004; 97(5): 1823 - 1829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Power and K. D. Patel Neurolipidomics: An inflammatory perspective on fat in the brain Neurology, August 24, 2004; 63(4): 608 - 609. [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Ivanova, S. B. Milne, J. S. Forrester, and H. A. Brown LIPID Arrays: New Tools in the Understanding of Membrane Dynamics and Lipid Signaling Mol. Interv., April 1, 2004; 4(2): 86 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Forrester, S. B. Milne, P. T. Ivanova, and H. A. Brown Computational Lipidomics: A Multiplexed Analysis of Dynamic Changes in Membrane Lipid Composition during Signal Transduction Mol. Pharmacol., April 1, 2004; 65(4): 813 - 821. [Abstract] [Full Text]
|
|
|
|
|
|
E. P. Newberry, Y. Xie, S. Kennedy, X. Han, K. K. Buhman, J. Luo, R. W. Gross, and N. O. Davidson Decreased Hepatic Triglyceride Accumulation and Altered Fatty Acid Uptake in Mice with Deletion of the Liver Fatty Acid-binding Protein Gene J. Biol. Chem., December 19, 2003; 278(51): 51664 - 51672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. DiNitto, T. C. Cronin, and D. G. Lambright Membrane Recognition and Targeting by Lipid-Binding Domains Sci. Signal., December 16, 2003; 2003(213): re16 - re16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Bazan Synaptic lipid signaling: significance of polyunsaturated fatty acids and platelet-activating factor J. Lipid Res., December 1, 2003; 44(12): 2221 - 2233. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
