HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M200429-JLR200v1 44/6/1132    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thuahnai, S. T. Articles by Phillips, M. C. Search for Related Content PubMed PubMed Citation Articles by Thuahnai, S. T. Articles by Phillips, M. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 44, 1132-1142, June 2003
Copyright © 2003 by Lipid Research, Inc.

A quantitative analysis of apolipoprotein binding to SR-BI

Stephen T. Thuahnai^*, Sissel Lund-Katz^*, G. M. Anantharamaiah, David L. Williams and Michael C. Phillips^1,*

^* Division of GI/Nutrition, Lipid Research Group, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-4318
Biochemistry and Molecular Genetics, Atherosclerosis Research Unit, University of Alabama at Birmingham, Birmingham, AL 35294
Department of Pharmacological Sciences, University Medical Center, State University of New York at Stony Brook, Stony Brook, NY 11794

¹ To whom correspondence should be addressed. e-mail: phillipsmi{at}email.chop.edu

Competitive binding experiments were performed using Y1-BS1 adrenal cells to provide information about the interaction of HDL apolipoproteins with scavenger receptor class B, type I (SR-BI). Exchangeable apolipoproteins apolipoprotein A-I (apoA-I), apoA-II, apoE-2, apoE-3, and apoE-4 as phospholipid complexes bind like HDL₃ to SR-BI via their multiple amphipathic -helices; the concentrations required to reduce the binding of HDL₃ to SR-BI by 50% (IC₅₀) were similar and in the range of 35–50 µg protein/ml. In the case of apoA-I, peptides corresponding to segments 1–85, 44–65, 44–87, 149–243, and 209–241 all had the same IC₅₀ as each other (P = 0.86), showing that a specific amino acid sequence in apoA-I is not responsible for the interaction with SR-BI. The distribution of charged residues in the amphipathic -helix affects the interaction, with class A and Y helices binding better than class G* helices. Synthetic -helical peptides composed of either L or D amino acids can bind equally to the receptor. Association with phospholipid increases the amount of apolipoprotein binding to SR-BI without altering the affinity of binding. Lipid-free apolipoproteins compete only partially with the binding of HDL to SR-BI, whereas lipidated apolipoproteins compete fully.

These results are consistent with the existence of more than one type of apolipoprotein binding site on SR-BI.

Abbreviations: CE, cholesteryl ester; CNBr, cyanogen bromide; DMPC, 1,2-dimyristoyl phosphatidylcholine; GdnHCl, guanidine hydrochloride; MLV, multilamellar vesicle; POPC, 1-palmitoyl-2-oleoyl phosphatidylcholine; rHDL, reconstituted HDL; SR-BI, scavenger receptor class B, type I; SUV, small unilamellar vesicle

Supplementary key words scavenger receptor class B, type I • lipoprotein metabolism • high density lipoprotein • lipoprotein receptor • amphipathic -helix

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Van Eck, M. Hoekstra, R. Out, I. S. T. Bos, J. K. Kruijt, R. B. Hildebrand, and T. J. C. Van Berkel Scavenger receptor BI facilitates the metabolism of VLDL lipoproteins in vivo J. Lipid Res., January 1, 2008; 49(1): 136 - 146. [Abstract] [Full Text] [PDF]

				S. Kurumada, A. Onishi, H. Imai, K. Ishii, T. Kobayashi, and S. B. Sato Stage-Specific Association of Apolipoprotein A-I and E in Developing Mouse Retina Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1815 - 1823. [Abstract] [Full Text] [PDF]

				A. H.E.M. Klerkx, K. E. Harchaoui, W. A. van der Steeg, S. M. Boekholdt, E. S.G. Stroes, J. J.P. Kastelein, and J. A. Kuivenhoven Cholesteryl Ester Transfer Protein (CETP) Inhibition Beyond Raising High-Density Lipoprotein Cholesterol Levels: Pathways by Which Modulation of CETP Activity May Alter Atherogenesis Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 706 - 715. [Abstract] [Full Text] [PDF]

				A. A. Ajees, G. M. Anantharamaiah, V. K. Mishra, M. M. Hussain, and H. M. K. Murthy Crystal structure of human apolipoprotein A-I: Insights into its protective effect against cardiovascular diseases PNAS, February 14, 2006; 103(7): 2126 - 2131. [Abstract] [Full Text] [PDF]

				B. F. Asztalos, M. de la Llera-Moya, G. E. Dallal, K. V. Horvath, E. J. Schaefer, and G. H. Rothblat Differential effects of HDL subpopulations on cellular ABCA1- and SR-BI-mediated cholesterol efflux J. Lipid Res., October 1, 2005; 46(10): 2246 - 2253. [Abstract] [Full Text] [PDF]

				N. Dashti, G. Datta, M. Manchekar, M. Chaddha, and G. M. Anantharamaiah Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells J. Lipid Res., October 1, 2004; 45(10): 1919 - 1928. [Abstract] [Full Text] [PDF]

				B. Zhang, P. Fan, E. Shimoji, H. Xu, K. Takeuchi, C. Bian, and K. Saku Inhibition of Cholesteryl Ester Transfer Protein Activity by JTT-705 Increases Apolipoprotein E-Containing High-Density Lipoprotein and Favorably Affects the Function and Enzyme Composition of High-Density Lipoprotein in Rabbits Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1910 - 1915. [Abstract] [Full Text] [PDF]

				D. Rhainds, P. Bourgeois, G. Bourret, K. Huard, L. Falstrault, and L. Brissette Localization and regulation of SR-BI in membrane rafts of HepG2 cells J. Cell Sci., July 1, 2004; 117(15): 3095 - 3105. [Abstract] [Full Text] [PDF]

				S. Parathath, D. Sahoo, Y. F. Darlington, Y. Peng, H. L. Collins, G. H. Rothblat, D. L. Williams, and M. A. Connelly Glycine 420 Near the C-terminal Transmembrane Domain of SR-BI Is Critical for Proper Delivery and Metabolism of High Density Lipoprotein Cholesteryl Ester J. Biol. Chem., June 11, 2004; 279(24): 24976 - 24985. [Abstract] [Full Text] [PDF]

				L. Krimbou, M. Denis, B. Haidar, M. Carrier, M. Marcil, and J. Genest Jr. Molecular interactions between apoE and ABCA1: impact on apoE lipidation J. Lipid Res., May 1, 2004; 45(5): 839 - 848. [Abstract] [Full Text] [PDF]

				M. C. de Beer, L. W. Castellani, L. Cai, A. J. Stromberg, F. C. de Beer, and D. R. van der Westhuyzen ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 J. Lipid Res., April 1, 2004; 45(4): 706 - 715. [Abstract] [Full Text] [PDF]

				S. T. Thuahnai, S. Lund-Katz, P. Dhanasekaran, M. de la Llera-Moya, M. A. Connelly, D. L. Williams, G. H. Rothblat, and M. C. Phillips Scavenger Receptor Class B Type I-mediated Cholesteryl Ester-selective Uptake and Efflux of Unesterified Cholesterol: INFLUENCE OF HIGH DENSITY LIPOPROTEIN SIZE AND STRUCTURE J. Biol. Chem., March 26, 2004; 279(13): 12448 - 12455. [Abstract] [Full Text] [PDF]

				D. Wustner, M. Mondal, A. Huang, and F. R. Maxfield Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells J. Lipid Res., March 1, 2004; 45(3): 427 - 437. [Abstract] [Full Text] [PDF]

				N. R. Webb, M. C. de Beer, F. C. de Beer, and D. R. van der Westhuyzen ApoB-containing lipoproteins in apoE-deficient mice are not metabolized by the class B scavenger receptor BI J. Lipid Res., February 1, 2004; 45(2): 272 - 280. [Abstract] [Full Text] [PDF]

				B. L. Trigatti, M. Krieger, and A. Rigotti Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1732 - 1738. [Abstract] [Full Text] [PDF]