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Journal of Lipid Research, Vol. 45, 148-154, January 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women

Kristina Linder^1,*, Peter Arner, Amilcar Flores-Morales^*, Petra Tollet-Egnell^* and Gunnar Norstedt^*

^* Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden
Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden

¹ To whom correspondence should be addressed. e-mail: kristina.linder{at}cmm.ki.se

There is growing evidence that the distribution of adipose tissue in the body is of importance in the development of metabolic complications of obesity, such as diabetes, hypertension, and hyperlipidemia. The aim of this study was to identify differentially expressed genes in subcutaneous and omental human adipose tissue in obese men, using a subtractive hybridization strategy. From the obtained set of differentially expressed transcripts, we also aimed to identify genes that have a sex-specific pattern of expression in omental or subcutaneous adipose tissue. Representational difference analysis (RDA) was performed on cDNA from subcutaneous and omental fat tissue from a man with extreme abdominal obesity. Forty-four putatively differentially expressed genes were identified. The obtained RDA products were spotted onto glass slides to screen for differential expression in other obese patients by using a microarray hybridization procedure. Five genes were confirmed to be differentially expressed in subcutaneous or omental adipose tissue from male or female obese patients. One gene was detected only in males and was found to be upregulated in subcutaneous tissue.

The findings extend previous knowledge that different fat depots have differential gene expression and indicate that sex differences exist in adipose gene expression patterns. Linder, K., P. Arner, A. Flores-Morales, P. Tollet-Egnell, and G. Norstedt. Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women. J. Lipid Res. 2004. 45: 148–154.

Supplementary key words representational difference analysis • differential gene expression • DNA microarray analysis • adipsin • ras • phospholipid transfer • calcyclin

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