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Journal of Lipid Research, Vol. 45, 214-222, February 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology
Department of Medicine, University of Innsbruck, Innsbruck, Austria
1 To whom correspondence should be addressed. e-mail: andreas.ritsch{at}uibk.ac.at
To further elucidate the role of scavenger receptor class B type I (SR-BI) in reverse cholesterol transport and in atherogenesis, we performed studies in the rabbit, an animal model displaying a lipoprotein profile similar to that of human, expressing cholesteryl ester transfer protein in plasma and having been demonstrated to be susceptible to atherosclerosis. In this report, we describe for the first time the isolation and characterization of rabbit cDNA fragments encoding SR-BI and scavenger receptor class B type II (SR-BII). Development of an isoform-specific Taqman Real Time PCR system and generation of isoform-specific polyclonal antibodies allowed us to measure SR-BI and SR-BII expression in various rabbit organs on mRNA and protein levels, respectively. We found the highest expression of SR-BI in adrenal gland, liver, and proximal intestine; lesser expression was found in appendix and spleen. Immunohistochemical staining of frozen sections showed SR-BI expression in the cortex but not in the medulla of adrenal gland. An increasing portal to central vein gradient of expression was found within the hepatic lobule.
As shown in this report, identification and characterization of SR-BI expression in the rabbit affords a powerful tool to elucidate the role of SR-BI in cholesterol homeostasis and atherogenesis in human.
Abbreviations: DIG, digoxigenin; HRP, horseradish peroxidase; RACE, rapid amplification of cDNA ends; SR-BI, scavenger receptor class B type I; SR-BII, scavenger receptor class B type II
Supplementary key words antibody • atherosclerosis • cDNA cloning • enzyme-linked immunosorbent assay • high density lipoprotein • immunohistochemical staining • real-time polymerase chain reaction • reverse cholesterol transport
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