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Journal of Lipid Research, Vol. 45, 736-741, April 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology
* Institute of Human Nutrition, University of Southampton, Southampton, SO16 7PX UK
Centre for Dairy Research, School of Food Biosciences, University of Reading, Reading, RG6 6AP, UK
Department of Agriculture, and Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, University of Reading, Reading, RG6 6AP, UK
1 To whom correspondence should be addressed. e-mail: g.c.burdge{at}soton.ac.uk
This study investigated the incorporation of cis-9,trans-11 conjugated linoleic acid (c9,t11 CLA) and trans-10,cis-12-CLA (t10,c12 CLA) into plasma and peripheral blood mononuclear cell (PBMC) lipids when consumed as supplements highly enriched in these isomers. Healthy men (n = 49, age 31 ± 8 years) consumed one, two, and four capsules containing 
600 mg of either c9,t11 CLA or t10,c12 CLA per capsule for sequential 8 week periods followed by a 6 week washout before consuming the alternative isomer. Both isomers were incorporated in a dose-dependent manner into plasma phosphatidylcholine (PC) (c9,t11 CLA r = 0.779, t10,c12 CLA r = 0.738; P < 0.0001) and cholesteryl ester (CE) (c9,t11 CLA r = 0.706, t10,c12 CLA r = 0.788; P < 0.0001). Only t10,c12 CLA was enriched in plasma nonesterified fatty acids. Both c9,t11 CLA and t10,c12 CLA were incorporated linearly into PBMC total lipids (r = 0.285 and r = 0.273, respectively; P < 0.0005). The highest concentrations of c9,t11 CLA and t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than those in plasma PC and CE.
These data suggest that the level of intake is a major determinant of plasma and PBMC CLA content, although PBMCs appear to incorporate both CLA isomers less readily.
Abbreviations: CE, cholesteryl ester; CLA, conjugated linoleic acid; FAME, fatty acid methyl ester; NEFA, nonesterified fatty acid; PBMC, peripheral blood mononuclear cell; PC, phosphatidylcholine
Supplementary key words phosphatidylcholine • cholesteryl ester • nonesterified fatty acids • leukocyte • human
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