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Journal of Lipid Research, Vol. 45, 873-880, May 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Oxidized LDL further enhances expression of adhesion molecules in Chlamydophila pneumoniae-infected endothelial cells

Silvana A. Vielma^*, Marina Mironova^*, Ja-Ran Ku^* and Maria F. Lopes-Virella^1,*,

^* Department of Medicine, Division of Endocrinology-Metabolism-Nutrition, Medical University of South Carolina, Charleston, SC
Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC

¹ To whom correspondence should be addressed. e-mail: virellam{at}musc.edu

Chlamydophila pneumoniae is a common respiratory pathogen that has been shown to be associated with coronary artery disease. Recent studies have shown that one of the possible mechanisms of the atherogenicity of C. pneumoniae is overexpression of cell adhesion molecules (CAMs) in infected endothelial cells. We investigated whether exposure of C. pneumoniae-infected endothelial cells to oxidized LDL (oxLDL) leads to further upregulation of CAMs. Flow cytometry and immunoblot analysis of human aortic endothelial cells (HAECs) was performed for intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. ICAM-1 was expressed in 78.7% of C. pneumoniae-infected HAECs. The addition of oxLDL (100 µg/ml) to infected HAECs increased the proportion of ICAM-1-positive cells to 92%. VCAM-1 was only observed in 9.3% of infected HAECs, and the addition of oxLDL had no further effect on the surface expression of VCAM-1. C. pneumoniae also upregulated the surface expression of E-selectin on 52.2% of the cells, and incubation with oxLDL further increased the proportion of positive cells to 63.64%.

In conclusion, C. pneumoniae upregulated the expression of the adhesion molecules ICAM-1, VCAM-1, and E-selectin on HAECs. The addition of oxLDL to the infected cells further enhanced the surface expression of ICAM-1 and E-selectin.

Supplementary key words intracellular adhesion molecule-1 • E-selectin • vascular cell adhesion molecule-1 • oxidized low density lipoprotein • cell adhesion molecules

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