Plasma phosphatidylcholine hydroperoxide as a new marker of oxidative stress in alcoholic patients

Junko Adachi¹^,*, Sachio Matsushita, Naoki Yoshioka^*, Rika Funae^*, Tetsuo Fujita^*, Susumu Higuchi and Yasuhiro Ueno^*

^* Department of Legal Medicine, Kobe University Graduate School of Medicine, 7 Kusunoki-cho Chuo-ku, Kobe, 650-0017 Japan
National Institute on Alcoholism, Kurihama National Hospital, 5 Nobi Yokosuka, Kanagawa, 239-0841 Japan

^¹ To whom correspondence should be addressed. e-mail: adachi{at}med.kobe-u.ac.jp

Quantitative analysis of plasma phosphatidylcholine hydroperoxide(PCOOH) is an important step in evaluating the biochemical processesleading to oxidative injury. However, secondary products oflipid peroxidation are now used as indices. One hundred ninealcoholic patients, aged 22–81 years (mean ± SEM,52.0 ± 1.3 years), and 21 healthy volunteers, aged 41–79years (51.2 ± 2.2 years), participated in this study.Plasma PCOOH was measured by HPLC with chemiluminescence detection.Plasma PCOOH concentration was significantly higher in alcoholicpatients (46.1 ± 4.1 pmol/ml) than in controls (15.6± 1.8 pmol/ml). It was significantly higher in patientswith blood alcohol (88.0 ± 10.5 pmol/ml) than in thosewithout alcohol (32.6 ± 3.1 pmol/ml). The patients withhigh levels of aspartate aminotransferase, alanine aminotransferase, ${gamma}$ -glutamyl transpeptidase ( ${gamma}$ -GTP), and triglyceride (TG) showedsignificantly higher PCOOH concentrations than did patientswith normal levels. The PCOOH level was positively correlatedwith levels of ${gamma}$ -GTP, HDL, blood alcohol concentration, and TG.Plasma PCOOH levels in 29 alcoholic patients after a 6 weekabstinence were decreased significantly (22.8 ± 11.1pmol/ml), which was associated with improvement on liver functiontests. This is the first measurement of plasma PCOOH in alcoholicpatients.

These results suggest the involvement of lipid peroxidationin alcohol-induced liver damage and confirm that the PCOOH plasmaconcentration is a new marker of alcohol consumption as wellas oxidative stress in alcoholic patients.

Abbreviations: ALD, alcoholic liver disease; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BHT, 3,5-di-tert-butyl-4-hydroxytoluene; CL, chemiluminescence detection; ${gamma}$ -GTP, ${gamma}$ -glutamyl transpeptidase; PCOOH, phosphatidylcholine hydroperoxide; TC, total cholesterol; TG, triglyceride

Supplementary key words high-performance liquid chromatography • liver disorder • lipid peroxidation

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