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Originally published In Press as doi:10.1194/jlr.M400003-JLR200 on May 16, 2004

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Journal of Lipid Research, Vol. 45, 1468-1474, August 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x

Que Lan1,* and Randall J. Massey{dagger}

* Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706
{dagger} Electron Microscope Facility, University of Wisconsin-Madison, Madison, WI 53706

1 To whom correspondence should be addressed. e-mail: qlan{at}entomology.wisc.edu

Subcellular distribution of Aedes aegypti sterol carrier protein-2 (AeSCP-2) and AeSCP-x was studied using electron microscopy. In both cultured A. aegypti cells and in the larval midgut, AeSCP-2 was detected mostly in the cytosol, with some labeling mitochondria and nucleus, but not in membranous vesicles. The widespread distribution of AeSCP-2 in the midgut epithelium is consistent with its potential lipid transfer function in all phases of cholesterol absorption. In contrast, AeSCP-x was found mostly in the peroxisome. Differences in the subcellular distribution of AeSCP-2 and AeSCP-x suggest that these two members of the SCP-2 gene family are functionally distinct. Overexpression of AeSCP-2 in A. aegypti cells showed increased localization of AeSCP-2 to cytosol, mitochondria, and nucleus. This is the first report on the nuclear distribution of an SCP.

Overexpression of AeSCP-2 resulted in increased cholesterol incorporation in cells, suggesting that AeSCP-2 enhances cholesterol uptake.

Abbreviations: Aag-2, Aedes aegypti cell line-2; AeSCP-2, Aedes aegypti sterol carrier protein-2; AeSCP-xC, AeSCP-x C-terminal; dsRNA, double-stranded RNA; RNAi, RNA interference

Supplementary key words Aedes aegypti cell line-2 • cholesterol • immunoelectron microscopy • overexpression • ribonucleic acid interference


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