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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M500185-JLR200 on August 1, 2005

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Journal of Lipid Research, Vol. 46, 2295-2298, October 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Methods

Separation of polyprenol and dolichol by monolithic silica capillary column chromatography

Takeshi Bamba*,{dagger}, Eiiciro Fukusaki1,*, Hiroshi Minakuchi§, Yoshihisa Nakazawa{dagger} and Akio Kobayashi*

* Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
{dagger} Hitachi Zosen Corporation, 2-2-11 Funamachi, Taisyou-ku, Osaka 551-0022, Japan
§ Kyoto Monotech, 13 Shimotsubayashi Shibanomiya-cho, Nishikyo-ku, Kyoto, Kyoto 615- 8035, Japan

Published, JLR Papers in Press, August 1, 2005. DOI 10.1194/jlr.M500185-JLR200

1 To whom correspondence should be addressed. e-mail: fukusaki{at}bio.eng.osaka-u.ac.jp

We attempted an analysis of naturally occurring polyprenol and dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mixture alone was performed using a 250 x 0.2 mm inner diameter (ID) octadecylsilyl (ODS)-monolithic silica capillary column. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by ≥2-fold the level recorded when using a conventional ODS-silica particle-packed column (250 x 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and dolichol type (dihydropolyprenol) was subjected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analysis of polyprenol fraction derived from Eucommia ulmoides leaves, dolichols were found as a single peak, including all-trans-polyprenol and cis-polyprenol previously identified.

This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polyprenols and dolichols and that have a low content.

Abbreviations: ODS, octadecylsilyl; SFC, supercritical fluid chromatography

Supplementary key words high-performance liquid chromatography • profiling • metabolomics • metabolome


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