J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M500054-JLR200 on April 16, 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500054-JLR200v1
46/7/1517    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gauster, M.
Right arrow Articles by Frank, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gauster, M.
Right arrow Articles by Frank, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 46, 1517-1525, July 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Endothelial lipase releases saturated and unsaturated fatty acids of high density lipoprotein phosphatidylcholine

M. Gauster*, G. Rechberger{dagger}, A. Sovic*, G. Hörl*, E. Steyrer*, W. Sattler* and S. Frank1,*

* Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Harrachgasse 21/III, Graz A-8010, Austria
{dagger} Institute of Molecular Biology, Biochemistry and Microbiology, University of Graz, Heinrichstrasse 31a, Graz A-8010, Austria

Published, JLR Papers in Press, April 16, 2005. DOI 10.1194/jlr.M500054-JLR200

1 To whom correspondence should be addressed. e-mail: sasa.frank{at}meduni-graz.at

We assessed the ability of endothelial lipase (EL) to hydrolyze the sn-1 and sn-2 fatty acids (FAs) from HDL phosphatidylcholine. For this purpose, reconstituted discoidal HDLs (rHDLs) that contained free cholesterol, apolipoprotein A-I, and either 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or 1-palmitoyl-2-arachidonylphosphatidylcholine were incubated with EL- and control (LacZ)-conditioned media. Gas chromatography analysis of the reaction mixtures revealed that both the sn-1 (16:0) and sn-2 (18:1, 18:2, and 20:4) FAs were liberated by EL. The higher rate of sn-1 FA cleavage compared with sn-2 FA release generated corresponding sn-2 acyl lyso-species as determined by MS analysis. EL failed to release sn-2 FA from rHDLs containing 1-O-1'-hexadecenyl-2-arachidonoylphosphatidylcholine, whose sn-1 position contained a nonhydrolyzable alkyl ether linkage.

The lack of phospholipase A2 activity of EL and its ability to liberate [14C]FA from [14C]lysophosphatidylcholine (lyso-PC) led us to conclude that EL-mediated deacylation of phosphatidylcholine (PC) is initiated at the sn-1 position, followed by the release of the remaining FA from the lyso-PC intermediate. Thin-layer chromatography analysis of cellular lipids obtained from EL-overexpressing cells revealed a pronounced accumulation of [14C]phospholipid and [14C]triglyceride upon incubation with 1-palmitoyl-2-[1-14C]linoleoyl-PC-labeled HDL3, indicating the ability of EL to supply cells with unsaturated FAs.

Abbreviations: DMEM, Dulbecco's modified Eagle's medium; EL, endothelial lipase; EL-Ad, adenovirus encoding human endothelial lipase; FA, fatty acid; FCS, fetal calf serum; FFA, free fatty acid; GC, gas chromatography; HDL-PC, HDL phosphatidylcholine; LacZ-Ad, adenovirus encoding ß-galactosidase; lyso-PC, lysophosphatidylcholine; MOI, multiplicity of infection; PAPC, 1-palmitoyl-2-arachidonylphosphatidylcholine; PL, phospholipid; plasmalogen-PC, 1-O-1'-hexadecenyl-2-arachidonoylphosphatidylcholine; PLPC, 1-palmitoyl-2-linoleoylphosphatidylcholine; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; TG, triglyceride; TLC, thin-layer chromatography

Supplementary key words phospholipases • lysophospholipases • phospholipids • polyunsaturated fatty acid • reconstituted high density lipoprotein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
M. Gauster, U. Hiden, A. Blaschitz, S. Frank, U. Lang, G. Alvino, I. Cetin, G. Desoye, and C. Wadsack
Dysregulation of Placental Endothelial Lipase and Lipoprotein Lipase in Intrauterine Growth-Restricted Pregnancies
J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2256 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
G. Qiu, A. C. Ho, W. Yu, and J. S. Hill
Suppression of endothelial or lipoprotein lipase in THP-1 macrophages attenuates proinflammatory cytokine secretion
J. Lipid Res., February 1, 2007; 48(2): 385 - 394.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. L. Magnusson-Olsson, B. Hamark, A. Ericsson, M. Wennergren, T. Jansson, and T. L. Powell
Gestational and hormonal regulation of human placental lipoprotein lipase
J. Lipid Res., November 1, 2006; 47(11): 2551 - 2561.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Jin, I. V. Fuki, N. G. Seidah, S. Benjannet, J. M. Glick, and D. J. Rader
Proprotein Covertases Are Responsible for Proteolysis and Inactivation of Endothelial Lipase
J. Biol. Chem., November 4, 2005; 280(44): 36551 - 36559.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.