HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: D500014-JLR200v1 46/8/1773    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by El Hamidi, A. Articles by Caroff, M. Search for Related Content PubMed PubMed Citation Articles by El Hamidi, A. Articles by Caroff, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 46, 1773-1778, August 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Methods

Microextraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization

Asmaa El Hamidi^*, Alina Tirsoaga^*,, Alexey Novikov^*, Ahmed Hussein^* and Martine Caroff^1,*

^* Equipe Endotoxines, Unité Mixte de Recherche 8619 du Centre National de la Recherche Scientifique, Institut de Biochimie, Biophysique Moléculaire et Cellulaire, Université de Paris-Sud, 91405 Orsay, France
Department of Physical Chemistry, University of Bucharest, Bucharest, Romania

Published, JLR Papers in Press, June 1, 2005. DOI 10.1194/jlr.D500014-JLR200

¹ To whom correspondence should be addressed. e-mail: martine.caroff{at}bbmpc.u-psud.fr

Endotoxins (lipopolysaccharides) are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures, which vary greatly with cell growth conditions.

This method is suitable for rough- and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.

Abbreviations: Kdo, 2-keto-3-deoxyoctonate; MALDI, matrix-assisted laser desorption/ionization; LPS, lipopolysaccharide; OS, oligosaccharide; PS, polysaccharide

Supplementary key words endotoxin • lipopolysaccharide • hydrolysis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Marr, A. Tirsoaga, D. Blanot, R. Fernandez, and M. Caroff Glucosamine Found as a Substituent of Both Phosphate Groups in Bordetella Lipid A Backbones: Role of a BvgAS-Activated ArnT Ortholog J. Bacteriol., June 15, 2008; 190(12): 4281 - 4290. [Abstract] [Full Text] [PDF]

				A. Tirsoaga, A. El Hamidi, M. B. Perry, M. Caroff, and A. Novikov A rapid, small-scale procedure for the structural characterization of lipid A applied to Citrobacter and Bordetella strains: discovery of a new structural element J. Lipid Res., November 1, 2007; 48(11): 2419 - 2427. [Abstract] [Full Text] [PDF]