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Journal of Lipid Research, Vol. 47, 78-86, January 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Cell surface-expressed moesin-like HDL/apoA-I binding protein promotes cholesterol efflux from human macrophages

Akifumi Matsuyama^*, Naohiko Sakai, Hisatoyo Hiraoka, Ken-ichi Hirano and Shizuya Yamashita^,1

^* Medical Center for Translational Research, Osaka University Hospital, 2-15 Yamada-oka, Suita
Department of Cardiology, Osaka University Graduate School of Medicine, B5, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan

Published, JLR Papers in Press, October 26, 2005.

¹ To whom correspondence should be addressed. e-mail: shizu{at}imed2.med.osaka-u.ac.jp

HDL and its major component, apolipoprotein A-I (apoA-I), play a central role in reverse cholesterol transport. We recently reported the involvement of a glycosylphosphatidylinositol anchor (GPI anchor) in the binding of HDL and apoA-I on human macrophages, and purified an 80 kDa HDL/apoA-I binding protein. In the present study, we characterized the GPI-anchored HDL/apoA-I binding protein from macrophages. The HDL/apoA-I binding protein was purified from macrophages and digested with endopeptidase, and the resultant fragments were sequenced. Cholesterol efflux, flow cytometry, immunoblotting, and immunohistochemical analyses were performed to characterize the HDL/apoA-I binding protein. Two parts of seven amino acid sequences completely matched those of moesin. Flow cytometry, immunoblotting, and immunohistochemistry using anti-moesin antibody showed that the HDL/apoA-I binding protein was N-glycosylated and expressed on the cell surface. It was termed moesin-like protein. Treatment of macrophages with anti-moesin antibody blocked the binding of HDL/apoA-I and suppressed cholesterol efflux. The moesin-like protein was exclusively expressed on macrophages and was upregulated by cholesterol loading and cell differentiation. Our results indicate that the moesin-like HDL/apoA-I binding protein is specifically expressed on the surface of human macrophages and promotes cholesterol efflux from macrophages.—Matsuyama, A, N. Sakai, H. Hiraoka, K-i. Hirano, and S. Yamashita. Cell surface-expressed moesin-like HDL/apoA-I binding protein promotes cholesterol efflux from human macrophages.

Abbreviations: AcLDL, acetylated LDL; apoA-I, apolipoprotein A-I; GPI, glycosylphosphatidylinositol; IEF, isoelectric focusing; MIF, mean intensity of fluorescence; PI-PLC, phosphatidylinositol-specific phospholipase C

Supplementary key words apolipoprotein A-I • glycosylphosphatidylinositol-anchored protein • moesin-like protein • atherosclerosis

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