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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M500205-JLR200 on October 18, 2005

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Journal of Lipid Research, Vol. 47, 99-106, January 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Measuring VLDL-triglyceride turnover in humans using ex vivo-prepared VLDL tracer

Lars C. Gormsen*, Michael D. Jensen1,{dagger} and Søren Nielsen*,{dagger}

* Medical Department M (Endocrinology and Diabetes), Aarhus University Hospital, DK-8000 Aarhus, Denmark
{dagger} Endocrine Research Unit, Mayo Clinic, Rochester, MN 55905

Published, JLR Papers in Press, October 18, 2005.

1 To whom correspondence should be addressed. e-mail: jensen.michael{at}mayo.edu

There has been more interest in VLDL-triglyceride (TG) kinetics during the last decade. Unfortunately, robust measurement methods are elaborate and not readily available. Here, we describe a method using unique, ex vivo labeling of the fatty acid moiety of VLDL-TG followed by intravenous bolus infusion in the same person. We found that plasma disappearance of ex vivo-labeled VLDL-TG was comparable to that of in vivo-labeled VLDL-TG and that turnover rates can be safely estimated from the log linear decay of VLDL-TG specific activity. We found minor labeling of the plasma FFA (oleate) pool, which was largely attributable to coinfusion of free [14C]triolein; VLDL-TG did not contribute substantially to the plasma FFA pool. The plasma decay curve of VLDL-TG was not affected by the presence of tracer in the FFA pool, provided that the data from 2 h after the VLDL tracer bolus infusion was used. The FFA contamination problem was circumvented by minor modification of the VLDL-TG tracer preparation. The approach we describe should expand the opportunity to study processes that cannot be assessed if the FFA precursor pool is labeled. This method for VLDL-TG tracer preparation can allow measurement of VLDL turnover, tissue uptake of VLDL-TG, and oxidation of VLDL-TG.

Supplementary key words very low density lipoprotein • lipoproteins • free fatty acids


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