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Journal of Lipid Research, Vol. 47, 2101-2111, October 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Thematic Review

Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Lipidomics: a global approach to lipid analysis in biological systems

Andrew D. Watson¹

Department of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095

Published, JLR Papers in Press, August 10, 2006.

¹ To whom correspondence should be addressed. e-mail: awatson{at}mednet.ucla.edu

Lipids are water-insoluble molecules that have a wide variety of functions within cells, including: 1) maintenance of electrochemical gradients; 2) subcellular partitioning; 3) first- and second-messenger cell signaling; 4) energy storage; and 5) protein trafficking and membrane anchoring. The physiological importance of lipids is illustrated by the numerous diseases to which lipid abnormalities contribute, including atherosclerosis, diabetes, obesity, and Alzheimer's disease. Lipidomics, a branch of metabolomics, is a systems-based study of all lipids, the molecules with which they interact, and their function within the cell. Recent advances in soft-ionization mass spectrometry, combined with established separation techniques, have allowed the rapid and sensitive detection of a variety of lipid species with minimal sample preparation. A "lipid profile" from a crude lipid extract is a mass spectrum of the composition and abundance of the lipids it contains, which can be used to monitor changes over time and in response to particular stimuli. Lipidomics, integrated with genomics, proteomics, and metabolomics, will contribute toward understanding how lipids function in a biological system and will provide a powerful tool for elucidating the mechanism of lipid-based disease, for biomarker screening, and for monitoring pharmacologic therapy.

Supplementary key words inflammation • mass spectrometry • eicosanoids • lipid peroxidation • atherosclerosis

Abbreviations: APCI, atmospheric pressure chemical ionization; APPI, atmospheric pressure photoionization; CI, chemical ionization; COX, cyclooxygenase; EI, electron ionization; ESI, electrospray ionization; ESI-MS, electrospray ionization-mass spectrometry; LOX, lipoxygenase; LTA₄, leukotriene A₄; LXR, liver X receptor; MALDI, matrix-assisted laser desorption ionization; MRM, multiple reaction monitoring; MS/MS, tandem mass spectrometry; PC, phosphatidylcholine; PI, phosphatidylinositol; SPE, solid-phase extraction

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