HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600248-JLR200v1 47/11/2444    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ranganathan, G. Articles by Kern, P. A. Search for Related Content PubMed PubMed Citation Articles by Ranganathan, G. Articles by Kern, P. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 47, 2444-2450, November 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

The lipogenic enzymes DGAT1, FAS, and LPL in adipose tissue: effects of obesity, insulin resistance, and TZD treatment

Gouri Ranganathan^1,*,, Resat Unal^*,, Irina Pokrovskaya^*,, Aiwei Yao-Borengasser^*,, Bounleut Phanavanh^*,, Beata Lecka-Czernik^*,, Neda Rasouli^*, and Philip A. Kern^*,

^* Central Arkansas Veterans HealthCare System, University of Arkansas for Medical Sciences, Little Rock, AR 72205
Department of Medicine, Division of Endocrinology, University of Arkansas for Medical Sciences, Little Rock, AR 72205
Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72205

Published, JLR Papers in Press, August 7, 2006.

¹ To whom correspondence should be addressed. e-mail: ranganathangouri{at}uams.edu

Acyl-coenzyme A:diacylglycerol transferase (DGAT), fatty acid synthetase (FAS), and LPL are three enzymes important in adipose tissue triglyceride accumulation. To study the relationship of DGAT1, FAS, and LPL with insulin, we examined adipose mRNA expression of these genes in subjects with a wide range of insulin sensitivity (S_I). DGAT1 and FAS (but not LPL) expression were strongly correlated with S_I. In addition, the expression of DGAT1 and FAS (but not LPL) were higher in normal glucose-tolerant subjects compared with subjects with impaired glucose tolerance (IGT) (P < 0.005). To study the effects of insulin sensitizers, subjects with IGT were treated with pioglitazone or metformin for 10 weeks, and lipogenic enzymes were measured in adipose tissue. After pioglitazone treatment, DGAT1 expression was increased by 33 ± 10% (P < 0.05) and FAS expression increased by 63 ± 8% (P < 0.05); however, LPL expression was not altered. DGAT1, FAS, and LPL mRNA expression were not significantly changed after metformin treatment. The treatment of mice with rosiglitazone also resulted in an increase in adipose expression of DGAT1 by 2- to 3-fold, as did the treatment of 3T3 F442A adipocytes in vitro with thiazolidinediones. These data support a more global concept suggesting that adipose lipid storage functions to prevent peripheral lipotoxicity.

Supplementary key words diacylglycerol transferase • fatty acid synthetase • lipoprotein lipase • thiazolidinedione

Abbreviations: BMI, body mass index; DGAT, acyl-coenzyme A:diacylglycerol transferase; FAS, fatty acid synthetase; IGT, impaired glucose tolerance; NGT, normal glucose tolerance; S_I, insulin sensitivity; TZD, thiazolidinedione; WAT, white adipose tissue

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C.-L. E. Yen, S. J. Stone, S. Koliwad, C. Harris, and R. V. Farese Jr. Thematic Review Series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis J. Lipid Res., November 1, 2008; 49(11): 2283 - 2301. [Abstract] [Full Text] [PDF]

				S. Santosa, D. D Hensrud, S. B Votruba, and M. D Jensen The influence of sex and obesity phenotype on meal fatty acid metabolism before and after weight loss Am. J. Clinical Nutrition, October 1, 2008; 88(4): 1134 - 1141. [Abstract] [Full Text] [PDF]

				M. Prentki and S. R. M. Madiraju Glycerolipid Metabolism and Signaling in Health and Disease Endocr. Rev., October 1, 2008; 29(6): 647 - 676. [Abstract] [Full Text] [PDF]

				P. M. Rogers, N. Mashtalir, M. A. Rathod, O. Dubuisson, Z. Wang, K. Dasuri, S. Babin, A. Gupta, N. Markward, W. T. Cefalu, et al. Metabolically Favorable Remodeling of Human Adipose Tissue by Human Adenovirus Type 36 Diabetes, September 1, 2008; 57(9): 2321 - 2331. [Abstract] [Full Text] [PDF]

				J. Donkor, L. M. Sparks, H. Xie, S. R. Smith, and K. Reue Adipose Tissue Lipin-1 Expression Is Correlated with Peroxisome Proliferator-Activated Receptor {alpha} Gene Expression and Insulin Sensitivity in Healthy Young Men J. Clin. Endocrinol. Metab., January 1, 2008; 93(1): 233 - 239. [Abstract] [Full Text] [PDF]

				C. Hu, A. Dandapat, J. Chen, Y. Fujita, N. Inoue, Y. Kawase, K.-i. Jishage, H. Suzuki, T. Sawamura, and J. L. Mehta LOX-1 deletion alters signals of myocardial remodeling immediately after ischemia-reperfusion Cardiovasc Res, November 1, 2007; 76(2): 292 - 302. [Abstract] [Full Text] [PDF]