| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print April 1, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Lipid Research, Vol. 47, 745-754, April 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology
* Departments of Medicine and Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, and VA Greater Los Angeles Healthcare System, Los Angeles, CA 90073
Division of Cardiology, Department of Internal Medicine, University of California, Los Angeles, CA 90095
Lipomics Technologies, West Sacramento, CA 95691
The online version of this article (available at http://www.jlr.org) contains additional table and figure.
Published, JLR Papers in Press, January 25, 2006.
1 To whom correspondence should be addressed. e-mail: reuek{at}ucla.edu
Triglyceride synthesis in most mammalian tissues involves the sequential addition of fatty acids to a glycerol backbone, with unique enzymes required to catalyze each acylation step. Acylation at the sn-2 position requires 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) activity. To date, seven Agpat genes have been identified based on activity and/or sequence similarity, but their physiological functions have not been well established. We have generated a mouse model deficient in AGPAT6, which is normally expressed at high levels in brown adipose tissue (BAT), white adipose tissue (WAT), and liver. Agpat6-deficient mice exhibit a 25% reduction in body weight and resistance to both diet-induced and genetically induced obesity. The reduced body weight is associated with increased energy expenditure, reduced triglyceride accumulation in BAT and WAT, reduced white adipocyte size, and lack of adipose tissue in the subdermal region. In addition, the fatty acid composition of triacylglycerol, diacylglycerol, and phospholipid is altered, with proportionally greater polyunsaturated fatty acids at the expense of monounsaturated fatty acids. Thus, Agpat6 plays a unique role in determining triglyceride content and composition in adipose tissue and liver that cannot be compensated by other members of the Agpat family.
Supplementary key words acyltransferase • gene-trap • adipose tissue • energy expenditure • 1-acylglycerol-3-phosphate O-acyltransferase
Abbreviations: AGPAT, 1-acylglycerol-3-phosphate O-acyltransferase; BAT, brown adipose tissue; DAG, diacylglycerol; DGAT1, acyl-coenzyme A:diacylglycerol acyltransferase-1; GPAT, glycerol-3-phosphate acyltransferase; HF/HC, high-fat/high-carbohydrate; TAG, triacylglycerol; WAT, white adipose tissue
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. E. Gimeno and J. Cao Thematic Review Series: Glycerolipids. Mammalian glycerol-3-phosphate acyltransferases: new genes for an old activity J. Lipid Res., October 1, 2008; 49(10): 2079 - 2088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bionaz and J. J. Loor ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 Are the Most Abundant Isoforms in Bovine Mammary Tissue and Their Expression Is Affected by Stage of Lactation J. Nutr., June 1, 2008; 138(6): 1019 - 1024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Q. Chen, M.-S. Kuo, S. Li, H. H. Bui, D. A. Peake, P. E. Sanders, S. J. Thibodeaux, S. Chu, Y.-W. Qian, Y. Zhao, et al. AGPAT6 Is a Novel Microsomal Glycerol-3-phosphate Acyltransferase J. Biol. Chem., April 11, 2008; 283(15): 10048 - 10057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Nagle, L. Vergnes, H. DeJong, S. Wang, T. M. Lewin, K. Reue, and R. A. Coleman Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4, as the enzyme deficient in Agpat6-/- mice J. Lipid Res., April 1, 2008; 49(4): 823 - 831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. F. Chehab Minireview: Obesity and LipOdystrophy--Where Do the Circles Intersect? Endocrinology, March 1, 2008; 149(3): 925 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Hegele, T. R. Joy, S. A. Al-Attar, and B. K. Rutt Thematic review series: Adipocyte Biology. Lipodystrophies: windows on adipose biology and metabolism J. Lipid Res., July 1, 2007; 48(7): 1433 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K Agarwal, S. Sukumaran, R. Bartz, R. I Barnes, and A. Garg Functional characterization of human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 9: cloning, tissue distribution, gene structure, and enzymatic activity J. Endocrinol., June 1, 2007; 193(3): 445 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cao, J.-L. Li, D. Li, J. F. Tobin, and R. E. Gimeno Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis PNAS, December 26, 2006; 103(52): 19695 - 19700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lusis A thematic review series: systems biology approaches to metabolic and cardiovascular disorders J. Lipid Res., September 1, 2006; 47(9): 1887 - 1890. [Full Text] [PDF]
|
|
|
|
|
|
K. Reue and L. Vergnes Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Approaches to lipid metabolism gene identification and characterization in the postgenomic era J. Lipid Res., September 1, 2006; 47(9): 1891 - 1907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Beigneux, L. Vergnes, X. Qiao, S. Quatela, R. Davis, S. M. Watkins, R. A. Coleman, R. L. Walzem, M. Philips, K. Reue, et al. Agpat6--a novel lipid biosynthetic gene required for triacylglycerol production in mammary epithelium J. Lipid Res., April 1, 2006; 47(4): 734 - 744. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
