J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M500503-JLR200 on March 13, 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500503-JLR200v1
47/6/1176    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Braun, N. A.
Right arrow Articles by Swift, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braun, N. A.
Right arrow Articles by Swift, L. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 47, 1176-1186, June 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells

Nicole A. Braun*, Peter J. Mohler*, Karl H. Weisgraber{dagger}, Alyssa H. Hasty§, MacRae F. Linton**,{dagger}{dagger}, Patricia G. Yancey{dagger}{dagger}, Yan Ru Su{dagger}{dagger}, Sergio Fazio*,{dagger}{dagger} and Larry L. Swift1,*

* Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232
§ Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232
** Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232
{dagger}{dagger} Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232
{dagger} Gladstone Institute of Neurological Disease, Cardiovascular Research Institute, and Department of Pathology, University of California, San Francisco, CA 94141

Published, JLR Papers in Press, March 13, 2006.

1 To whom correspondence should be addressed. e-mail: larry.swift{at}vanderbilt.edu

We have investigated apolipoprotein E (apoE) recycling in Chinese hamster ovary (CHO) cells, a peripheral cell that does not produce lipoproteins or express apoE. Using a pulse-chase protocol in which cells were pulsed with 125I-apoE-VLDL and chased for different periods, ~30% of the apoE internalized during the pulse was resecreted within a 4 h chase in a relatively lipid-free state. The addition of lysosomotropic agents or brefeldin A had no effect on apoE recycling. Unlike previous results with hepatocytes and macrophages, neither apoA-I nor upregulation of ABCA1 stimulated apoE recycling. However, cyclodextrin, which extracts cholesterol from plasma membrane lipid rafts, increased recycling. Confocal studies revealed that apoE, internalized during a 1 h pulse, colocalizes with early endosomal antigen-1, Rab5, Rab11a, and lysobisphosphatidic acid but not with lysosomal-associated membrane protein-1. Colocalization of apoE and Rab11a persisted even after cells had been chased for 1 h, suggesting a pool of apoE within the endosomal recycling compartment (ERC). Our data suggest that apoE recycling in CHO cells is linked to cellular cholesterol removal via the ERC and phospholipid-containing acceptors in a pathway alternative to the ABCA1-apoA-I axis.

Abbreviations: apoE, apolipoprotein E; BFA, brefeldin A; ß-CD, 2-hydroxypropyl ß-cyclodextrin; CHO, Chinese hamster ovary; EEA1, early endosome antigen-1; ERC, endosomal recycling compartment; F12K, Kaighn's modification of Ham's F-12 medium; LAMP-1, lysosomal-associated membrane protein-1; LBPA, lysobisphosphatidic acid; LPDS, lipoprotein-deficient serum; LRP, low density lipoprotein receptor-related protein; LXR, liver X receptor; SR-BI, scavenger receptor class B type I

Supplementary key words cholesterol efflux • recycling endosomes • Rab5 • Rab11a • early endosomes • ß-cyclodextrin • ATP binding cassette transporter A1


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Kockx, W. Jessup, and L. Kritharides
Regulation of Endogenous Apolipoprotein E Secretion by Macrophages
Arterioscler. Thromb. Vasc. Biol., June 1, 2008; 28(6): 1060 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Fan, S. Qiu, C. D. Overton, P. G. Yancey, L. L. Swift, W. G. Jerome, M. F. Linton, and S. Fazio
Impaired Secretion of Apolipoprotein E2 from Macrophages
J. Biol. Chem., May 4, 2007; 282(18): 13746 - 13753.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.