J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M500559-JLR200 on April 21, 2006

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Journal of Lipid Research, Vol. 47, 1358-1365, July 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

C-terminal interactions of apolipoprotein E4 respond to the postprandial state

Sarada D. Tetali1,*, Madhu S. Budamagunta{dagger}, John C. Voss{dagger} and John C. Rutledge*

* Division of Endocrinology, Clinical Nutrition, and Vascular Medicine, Department of Internal Medicine, University of California, Davis, CA 95616
{dagger} Department of Biochemistry and Molecular Medicine, School of Medicine, University of California, Davis, CA 95616

Published, JLR Papers in Press, April 21, 2006.

1 To whom correspondence should be addressed. e-mail: skanakagiri{at}cdavis.edu

Increased triglyceride-rich lipoproteins (TGRLs) in the postprandial state are associated with atherosclerosis. We investigated whether the postprandial state induced structural changes at the apolipoprotein E4 (apoE4) C terminus, its principal lipid binding domain, using electron paramagnetic resonance (EPR) spectroscopy of a site-directed spin label attached to the cysteine of apoE4-W264C. Spin coupling between labels located in the C termini was followed after mixing with preprandial and postprandial human plasma samples. Our results indicate that postprandial plasma triggers a reorganization of the protein such that the dipolar broadening is diminished, indicating a reduction in C-terminal interaction. The loss of spectral broadening was directly correlated with an increase in postprandial plasma triglycerides and was reduced with delipidated plasma. The spin-labeled apoE4 displayed a lipid preference of VLDL > LDL > HDL in the preprandial and postprandial states. The apoE4 shift to VLDL during the postprandial state was accompanied by a loss in spectral broadening of the protein. These findings suggest that apoE4 associated with LDL maintains self-association via its C terminus and that this association is diminished in VLDL-associated protein. Lipolyzed TGRL reflected a depletion of the C-terminal interaction of apoE4. Addition of palmitate to VLDL gave a similar response as lipolyzed TGRL, suggesting that lipolysis products play a major role in reorganizing apoE4 during the postprandial state.

Supplementary key words site directed spin labeling • electron paramagnetic resonance • triglyceride-rich lipoproteins


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