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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M600519-JLR200 on January 31, 2007

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Journal of Lipid Research, Vol. 48, 1099-1107, May 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

mRNA expression and antilipolytic role of phosphodiesterase 4 in rat adipocytes in vitro

Hong Wang1,* and Neilé K. Edens2,{dagger}

* Interdisciplinary PhD Program in Nutrition, The Ohio State University, Columbus, OH 43210
{dagger} Ross Products Division, Abbott Laboratories, Columbus, OH 43215

Published, JLR Papers in Press, January 31, 2007.

1 Present address of H. Wang: Division of Gerontology, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201.

2 To whom correspondence should be addressed. e-mail: neile.edens{at}abbott.com

Adipocyte lipolysis is dependent on an increase in the intracellular concentration of cAMP. Intracellular phosphodiesterases (PDEs) hydrolyze cAMP and limit stimulation of lipolysis. In the present study, the mRNA expression of PDE4 subtypes and the antilipolytic role of PDE4 in rat adipocytes were investigated. Fragments encoding PDE4A (233 bp), PDE4B (786 bp), PDE4C (539 bp), and PDE4D (262 bp) sequences were amplified by RT-PCR. The mRNA expression of PDE4 subtypes (A, B, C, D) determined by real-time quantitative PCR was 7, 18.7, 18.9, and 7.2% relative to PDE3B. Inhibition of PDE4 by rolipram increased basal lipolysis and reversed in part prostaglandin E2 antilipolysis. The combination of PDE3 and PDE4 inhibitors synergistically reversed both prostaglandin E2 and phenylisopropyl adenosine antilipolysis. Stimulation of adipocytes with prostaglandin E2 increased total PDE activity and PDE3 activity measured by hydrolysis of 3[H]cAMP by the particulate fraction of adipocytes. The present study confirmed that mRNAs for all four PDE4 subtypes were expressed in rat adipocytes, with PDE4B and PDE4C predominant. Moreover, PDE4 not only limits the rate of basal lipolysis but also contributes to prostaglandin E2 antilipolysis in rat adipocytes.

Supplementary key words lipolysis • phosphodiesterase • prostaglandin E2

Abbreviations: ISO, isoproterenol; KRH medium, Krebs-Ringer's-HEPES medium; PDE, phosphodiesterase; PGE2, prostaglandin E2; PIA, phenylisopropyl adenosine; QPCR, real-time quantitative PCR


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