HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Papers In Press, published online ahead of print July 1, 2007
J. Lipid Res., doi:10.1194/jlr.C700008-JLR200

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: C700008-JLR200v1 48/7/1457    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ward, W. C. Articles by Simon, J. D. Search for Related Content PubMed PubMed Citation Articles by Ward, W. C. Articles by Simon, J. D. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 48, 1457-1462, July 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Short Communication

Identification and quantification of dolichol and dolichoic acid in neuromelanin from substantia nigra of the human brain

Weslyn C. Ward^1,*, Ziqiang Guan^1,, Fabio A. Zucca, Ruggero G. Fariello, Reza Kordestani, Luigi Zecca^2,, Christian R. H. Raetz^2, and John D. Simon^2,*,

^* Department of Chemistry, Duke University, Durham, NC
Department of Biochemistry, Duke University Medical Center, Durham, NC
Institute of Biomedical Technologies, Consiglio Nazionale delle Ricerche, Segrate, Italy

The online version of this article (available at http://www.jlr.org) contains supplemental data in the form of three figures and two schemes.

Published, JLR Papers in Press, April 19, 2007.

¹ W. C. Ward and Z. Guan contributed equally to this work.

² To whom correspondence should be addressed. e-mail: luigi.zecca{at}itb.cnr.it (L.Z.); raetz{at}biochem.duke.edu (C.R.H.R.); jsimon{at}duke.edu (J.D.S.)

ABSTRACT

Neuromelanin (NM) isolated from the substantia nigra of the human brain is found to contain a series of dolichoic acids (dol-CA) containing 14–20 isoprene units. This is the first observation of dol-CA in a natural system. Using internally spiked nor-dolichol and nor-dolichoic acid standards, the concentrations of dolichol (dol) and dol-CA present in NM were determined. Remarkably, dol was only four times as abundant as dol-CA in NM. The distribution of dol-CA chains lengths in NM also differed from that of dol, suggesting that the enzyme(s) responsible for the conversion of dol to dol-CA prefer a dolichol substrate containing 19 isoprene units.

Supplementary key words dolichyl • isoprenoid • Parkinson's disease • melanin

Abbreviations: dol, dolichol; dol-CA, dolichoic acid; LC/MS, liquid chromatography-mass spectrometry; NM, neuromelanin; SN, substantia nigra

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Zecca, C. Bellei, P. Costi, A. Albertini, E. Monzani, L. Casella, M. Gallorini, L. Bergamaschi, A. Moscatelli, N. J. Turro, et al. New melanic pigments in the human brain that accumulate in aging and block environmental toxic metals PNAS, November 11, 2008; 105(45): 17567 - 17572. [Abstract] [Full Text] [PDF]