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Journal of Lipid Research, Vol. 49, 473-480, February 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Methods

Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo

Klaus Strobel^1,*, Joerg van den Hoff^*, and Jens Pietzsch^*

^* Institute of Radiopharmacy, Research Center Dresden-Rossendorf, Dresden, Germany
Department of Nuclear Medicine, University of Technology, Dresden, Germany

Published, JLR Papers in Press, November 16, 2007.

¹ To whom correspondence should be addressed. e-mail: k.strobel{at}fzd.de

We describe a localized proton magnetic resonance spectroscopy (¹H-MRS) method for in vivo measurement of lipid composition in very small voxels (1.5 mm x 1.5 mm x 1.5 mm) in adipose tissue in mice. The method uses localized point-resolved spectroscopy to collect ¹H spectra from voxels in intra-abdominal white adipose tissue (WAT) and brown adipose tissue (BAT) deposits. Nonlinear least-squares fits of the spectra in the frequency domain allow for accurate calculation of the relative amount of saturated, monounsaturated, and polyunsaturated fatty acids. All spectral data are corrected for spin-spin relaxation. The data show BAT of NMRI mice to be significantly different from BAT of NMRI nu/nu mice in all aspects except for the fraction of monounsaturated fatty acids (FM); for WAT, only the FM is different. BAT and WAT of NMRI mice differ in the amount of saturated and diunsaturated fatty acids. This method provides a potential tool for studying lipid metabolism in small animal models of disease during the initiation, progression, and manifestation of obesity-related disorders in vivo. Our results clearly demonstrate that localized ¹H-MRS of adipose tissue in vivo is possible at high spatial resolution with voxel sizes down to 3.4 ml.

Supplementary key words adipocytes • spin-spin relaxation time T₂ • exponential decay • triglyceride • triacylglycerol • nuclear magnetic resonance

Abbreviations: AUC, area under the curve; BAT, brown adipose tissue; FD, fraction of diunsaturated fatty acids; FM, fraction of monounsaturated fatty acids; FOV, field of view; FP, fraction of polyunsaturated fatty acids; FS, fraction of saturated fatty acids; FT, fraction of triunsaturated fatty acids; MCL, mean chain length; MRS, magnetic resonance spectroscopy; NEX, number of repetitions; PRESS, point-resolved spectroscopy; TE, echo time; TR, repetition time; WAT, white adipose tissue; WPF, water proton fraction

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