HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M700561-JLR200v1 49/7/1445    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Tardy, A.-L. Articles by Morio, B. PubMed PubMed Citation Articles by Tardy, A.-L. Articles by Morio, B. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 49, 1445-1455, July 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Effects of trans MUFA from dairy and industrial sources on muscle mitochondrial function and insulin sensitivity

Anne-Laure Tardy^*,, Christophe Giraudet^*,, Paulette Rousset^*,, Jean-Paul Rigaudière^*,, Brigitte Laillet^*,, Sandrine Chalancon^*,, Jérôme Salles^*,, Olivier Loreau, Jean-Michel Chardigny^*, and Béatrice Morio^1,*,

^* INRA, UMR 1019 Nutrition Humaine, F-63122 Saint Genès Champanelle, France
Univ Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, F-63000 Clermont-Ferrand, France
Commissariat à l'Energie Atomique, iBi TecS, Service de Chimie Bioorganique et de Marquage, Gif sur Yvette, F-91191, Paris, France

This work was supported by the French National Agency for Research.

Published, JLR Papers in Press, March 29, 2008.

¹ To whom correspondence should be addressed. e-mail: morio{at}clermont.inra.fr

Epidemiological studies suggest that chronic consumption of trans MUFA may alter muscle insulin sensitivity. The major sources of dietary trans MUFA (dairy fat vs. industrially hydrogenated oils) have different isomeric profiles and thus probably different metabolic consequences. These effects may involve alterations in muscle mitochondrial oxidative capacity, which may in turn promote insulin resistance if fatty acid oxidation is reduced. We report that in Wistar rats, an 8 week diet enriched (4% of energy intake) in either dairy, industrial, or control MUFA did not alter insulin and glucose responses to an intraperitoneal glucose tolerance test (1g/kg). In C2C12 myotubes, vaccenic and elaidic acids did not modify insulin sensitivity compared with oleic acid. Furthermore, the ex vivo total, mitochondrial and peroxisomal oxidation rates of [1-¹⁴C]oleic, vaccenic, and elaidic acids were similar in soleus and tibialis anterior rat muscle. Finally, an 8 week diet enriched in either dairy or industrial trans MUFA did not alter mitochondrial oxidative capacity in these two muscles compared with control MUFA but did induce a specific reduction in soleus mitochondrial ATP and superoxide anion production (P < 0.01 vs. control). In conclusion, dietary trans MUFA of dairy or industrial origin have similar effects and do not impair muscle mitochondrial capacity and insulin sensitivity.

Supplementary key words nutrition • diabetes • lipid metabolism • energy metabolism • dairy fat • hydrogenated oil

Abbreviations: COX, cytochrome C oxidase; CS, citrate synthase; DF, dairy fat; IF, industrial fat; IPGTT, intraperitoneal glucose tolerance test; MSR, mitochondrial superoxide anion radical; ROS, reactive oxygen species

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?